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Western Blot (WB) (Western blot analysis using KRT15 mouse mAb against A431 cell lysate.)

Mouse anti-Human KRT15 Monoclonal Antibody | anti-KRT15 antibody

Anti-KRT15 Mouse mAb

Gene Names
KRT15; K15; CK15; K1CO
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Synonyms
KRT15; Monoclonal Antibody; Anti-KRT15 Mouse mAb; K15; CK15; K1CO; KRT1; anti-KRT15 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
6E7
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
456
Applicable Applications for anti-KRT15 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Immunogen
Purified recombinant fragment of KRT15 expressed in E Coli.

Western Blot (WB)

(Western blot analysis using KRT15 mouse mAb against A431 cell lysate.)

Western Blot (WB) (Western blot analysis using KRT15 mouse mAb against A431 cell lysate.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HepG2 (left) and PACN-1 (right) cells using KRT15 mouse mAb (green). Red)

Immunofluorescence (IF) (Immunofluorescence analysis of HepG2 (left) and PACN-1 (right) cells using KRT15 mouse mAb (green). Red)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of PACN-1 cells using KRT15 mouse mAb (green) and negative control (purple).)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of PACN-1 cells using KRT15 mouse mAb (green) and negative control (purple).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using KRT15 mouse mAb)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using KRT15 mouse mAb)
Related Product Information for anti-KRT15 antibody
Entrez Summary: The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 15 is a type I keratin without a defined type II partner. Keratin 15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Expression of keratin 15 is downregulated in some hyperproliferating situations, such as psoriasis and hypertrophic scars. Because keratinocytes in psoriasis and hypertrophic scars are activated, it is suggested that keratin 15 expression is not compatible with keratinocyte activation and the keratin 15 gene is downregulated to maintain the activated phenotype.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49
NCBI Official Full Name
keratin, type I cytoskeletal 15
NCBI Official Synonym Full Names
keratin 15
NCBI Official Symbol
KRT15
NCBI Official Synonym Symbols
K15; CK15; K1CO
NCBI Protein Information
keratin, type I cytoskeletal 15
UniProt Protein Name
Keratin, type I cytoskeletal 15
Protein Family
UniProt Gene Name
KRT15
UniProt Synonym Gene Names
KRTB; CK-15; K15

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. [provided by RefSeq, Jul 2008]

Uniprot Description

K15: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: nucleus

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: epidermis development

Research Articles on KRT15

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Product Notes

The KRT15 krt15 (Catalog #AAA475966) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-KRT15 Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KRT15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the KRT15 krt15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT15, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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