Western Blot (WB)
(KRAS monoclonal antibody (M02), clone 4F3 Western Blot analysis of KRAS expression in HeLa (Cat # L013V1).)
Mouse KRAS Monoclonal Antibody | anti-KRAS antibody
KRAS (v-Ki-ras2 Kirsten rat Sarcoma Viral Oncogene Homolog, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2) (FITC)
Gene Names
KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
Applications
Western Blot
Purity
Purified
Synonyms
KRAS; Monoclonal Antibody; KRAS (v-Ki-ras2 Kirsten rat Sarcoma Viral Oncogene Homolog; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS3; RASK2) (FITC); v-Ki-ras2 Kirsten rat Sarcoma Viral Oncogene Homolog; RASK2; anti-KRAS antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4F3
Specificity
Recognizes KRAS.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Applicable Applications for anti-KRAS antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KRAS (NP_004976, 16aa-125aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
KSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTV
Conjugate
FITC
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(KRAS monoclonal antibody (M02), clone 4F3 Western Blot analysis of KRAS expression in HeLa (Cat # L013V1).)
Western Blot (WB)
(KRAS monoclonal antibody (M02), clone 4F3 Western Blot analysis of KRAS expression in HeLa (Cat # L013V1).)
Western Blot (WB)
(Western Blot analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M02), clone 4F3.Lane 1: KRAS transfected lysate (21 KDa).Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M02), clone 4F3.Lane 1: KRAS transfected lysate (21 KDa).Lane 2: Non-transfected lysate.)
Testing Data
(Detection limit for recombinant GST tagged KRAS is approximately 0.3ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged KRAS is approximately 0.3ng/ml as a capture antibody.)
Related Product Information for anti-KRAS antibody
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq]
Product Categories/Family for anti-KRAS antibody
References
1. Synergistic Effects of Combined Wnt/KRAS Inhibition in Colorectal Cancer Cells. Mologni L, Brussolo S, Ceccon M, Gambacorti-Passerini C.PLoS One. 2012;7(12):e51449. doi: 10.1371/journal.pone.0051449. Epub 2012 Dec 5.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,425 Da
NCBI Official Full Name
GTPase KRas isoform b
NCBI Official Synonym Full Names
Kirsten rat sarcoma viral oncogene homolog
NCBI Official Symbol
KRAS
NCBI Official Synonym Symbols
NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
NCBI Protein Information
GTPase KRas; K-Ras 2; K-ras p21 protein; PR310 c-K-ras oncogene; c-Ki-ras; c-Kirsten-ras protein; cellular c-Ki-ras2 proto-oncogene; oncogene KRAS2; transforming protein p21; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog
UniProt Protein Name
GTPase KRas
Protein Family
UniProt Gene Name
KRAS
UniProt Synonym Gene Names
KRAS2; RASK2
UniProt Entry Name
RASK_HUMAN
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Product Notes
The KRAS kras (Catalog #AAA6176254) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's KRAS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the KRAS kras for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRAS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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