anti-Mouse, Human KLRG1 Monoclonal Antibody
Hamster Anti-Mouse KLRG1/MAFA-LE/AF
Reactivity
Mouse, Human
Applications
Flow Cytometry, Immunoprecipitation
Synonyms
KLRG1; Monoclonal Antibody; Hamster Anti-Mouse KLRG1/MAFA-LE/AF; Hamster Anti-Mouse KLRG1/MAFA - Low Endotoxin/Azide-Free; Hamster Anti-Mouse KLRG1; MAFA; killer cell lectin-like receptor G1; mast cell function-associated-antigen; anti-KLRG1 antibody
Host
Syrian Hamster
Reactivity
Mouse, Human
Clonality
Monoclonal
Isotype
Hamster (Syrian) IgG2
Clone Number
2F1
Specificity
Mouse/Human KLRG1; Mr 30-38 kDa
Form/Format
Low Endotoxin, Azide - Free, (LE/AF)
Applicable Applications for anti-KLRG1 antibody
Flow Cytometry; Immunoprecipitation
Application Notes
FC – Quality Tested 1-21
IP-Reported in literature 1,2
WB-NR- Reported in literature2
WORKING DILUTIONS
Flow Cytometry
Purified (UNLB ) antibody <=1 ug/106cells
FITC and BIOT conjugates <= 1ug/106cells
PE conjugate <=0.2 ug/10 6cells
For Flow Cytometry, the suggested use of these reagents is in a final volume of 100 uL.
Since applications vary, you should determine the optimum working dilution for the product that is appropiate for your specific need.
IP-Reported in literature 1,2
WB-NR- Reported in literature2
WORKING DILUTIONS
Flow Cytometry
Purified (UNLB ) antibody <=1 ug/106cells
FITC and BIOT conjugates <= 1ug/106cells
PE conjugate <=0.2 ug/10 6cells
For Flow Cytometry, the suggested use of these reagents is in a final volume of 100 uL.
Since applications vary, you should determine the optimum working dilution for the product that is appropiate for your specific need.
Immunogen
IL-2-activated NK cells (A-LAK cells) from B6 (H-2b) mice
Characterization
To ensure acceptable performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.
Note
Some reagents contain sodium azide.
Preparation and Storage
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20°C.
Ships with Dry Ice.
Ships with Dry Ice.
Related Product Information for anti-KLRG1 antibody
The monoclonal antibody 2F1 reacts with mouse killer cell lectin-like receptor G1 (KLRG1; formerly known as mouse MAFA or 2F1-Ag), a homodimeric member of the lectin-like type 2 transmembrane receptor family that contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains.1, 2 These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1.3, 4 The 2F1 antibody stains 30-60% of NK1.1+CD3- splenocytes, and a small fraction of T cells in all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL).1, 5 Cell surface expression of KLRG1 is up-regulated by expression of MHC class I molecules. The effect of MHC class I expression is indirect, and can be mediated by interactions with class I-specific Ly49 inhibitory receptors.
References
1. Corral, L., T. Hanke, R.E. Vance, D. Cado, and D.H. Raulet. 2000. Eur. J. Immunol. 30:920.
2. Hanke, T., L. Corral, R.E., Vance, and D.H. Raulet. 1998. 28:4409.
3. Burshtyn, D., A. Scharenberg, N. Wagtmann, S. Rajagopalan, M. Peruzzi, J.-P. Kinet, and E.O. Long. 1997. Immunity 4:77.
4. Nakamura, M.C., E.C. Niemi, M.J. Fisher, L.D. Schultz, W.E. Seaman, and J.C. Ryan. 1997. J. Exp. Med. 185:673.
5. Liu, S., and D.H. Raulet. Personal communication.
2. Hanke, T., L. Corral, R.E., Vance, and D.H. Raulet. 1998. 28:4409.
3. Burshtyn, D., A. Scharenberg, N. Wagtmann, S. Rajagopalan, M. Peruzzi, J.-P. Kinet, and E.O. Long. 1997. Immunity 4:77.
4. Nakamura, M.C., E.C. Niemi, M.J. Fisher, L.D. Schultz, W.E. Seaman, and J.C. Ryan. 1997. J. Exp. Med. 185:673.
5. Liu, S., and D.H. Raulet. Personal communication.
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Product Notes
The KLRG1 (Catalog #AAA673580) is an Antibody produced from Syrian Hamster and is intended for research purposes only. The product is available for immediate purchase. The Hamster Anti-Mouse KLRG1/MAFA-LE/AF reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's KLRG1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunoprecipitation. FC – Quality Tested 1-21 IP-Reported in literature 1,2 WB-NR- Reported in literature2 WORKING DILUTIONS Flow Cytometry Purified (UNLB ) antibody <=1 ug/106cells FITC and BIOT conjugates <= 1ug/106cells PE conjugate <=0.2 ug/10 6cells For Flow Cytometry, the suggested use of these reagents is in a final volume of 100 uL. Since applications vary, you should determine the optimum working dilution for the product that is appropiate for your specific need. Researchers should empirically determine the suitability of the KLRG1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLRG1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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