Highly validated and characterized monoclonal/polyclonal
antibodies and recombinant
proteins
The majority of AAA Biotech’s antibodies are highly validated and can be use in multiple
applications such as ELISA, FC,
ICC, IF, IHC, IP, WB, etc. We have antibodies available for rare species, in multiple conjugated
forms or recombinant
antibodies.
As for our high quality proteins, the majority have 90% purity, detected by SDS-PAGE while some are
available in
different tags such as Flag, GST, His, MBP, etc. We also carry high quality native and biologically
active proteins.
AAA Biotech is constantly working to expand our capacity to provide recombinant proteins and
antibodies to most
target proteins.
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '21319'
AND `pd`.`language_id` = 1
LIMIT 1
Query
Database
1.86 ms
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '21319' and pd.language_id = 1
Query
Database
1.53 ms
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '21319'
Database (4 total Queries, 4 of them unique across 2 Connections)
Time
Query String
3.74 ms
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '21319'
AND `pd`.`language_id` = 1
LIMIT 1
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '21319' and pd.language_id = 1
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '21319'
IHC: 1:50-1:200<br>IHC-P: 1:200<br>WB: 1:500-1:2000<br>The predicted MW is 32kDa, while the observed MW by Western blot was 33kDa.
⇄⧉testing_protocols => string (1070) "IHC (Immunohistchemistry)||TNFRSF6B/DCR3 Antibody-Immunohistochemistry of pa...
$value['testing_protocols']
IHC (Immunohistchemistry)||TNFRSF6B/DCR3 Antibody-Immunohistochemistry of paraffin-embedded rat kidney tissue at 1:200 dilution.||AAA21319_IHC6.jpg!!IHC (Immunohistochemistry)||TNFRSF6B/DCR3 Antibody-Immunohistochemistry of paraffin-embedded human kidney cancer tissue at 1:200 dilution.||AAA21319_IHC5.jpg!!IHC (Immunohistochemistry)||TNFRSF6B/DCR3 Antibody-Immunohistochemistry of paraffin-embedded human normal stomach tissue at 1:200 dilution.||AAA21319_IHC4.jpg!!IHC (Immunohistochemistry)||TNFRSF6B/DCR3 Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)||AAA21319_IHC3.jpg!!WB (Western Blot)||TNFRSF6B/DCR3 Antibody-Western blot analysis of extracts of various cells.||AAA21319_WB2.jpg!!WB (Western Blot)||TNFRSF6B/DCR3 Antibody-Western blot analysis of extracts of HT-29 cells, using TNFRSF6B antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection.||AAA21319_WB.jpg
⇄⧉etc_term1 => string (473) "Target||Human TNFRSF6B/DCR3!!Immunogen||Recombinant fusion protein containin...
$value['etc_term1']
Target||Human TNFRSF6B/DCR3!!Immunogen||Recombinant fusion protein containing a sequence corresponding to amino acids 35-300 of human TNFRSF6B (NP_003814.1). TYPWRDAETGERLVCAQCPPGTFVQRPCRRDSPTTCGPCPPRHYTQFWNYLERCRYCNVLCGEREEEARACHATHNRACRCRTGFFAHAGFCLEHASCPPGAGVIAPGTPSQNTQCQPCPPGTFSASSSSSEQCQPHRNCTALGLALNVPGSSSHDTLCTSCTGFPLSTRVPGAEECERAVIDFVAFQDISIKRLQRLLQALEAPEGWGPTPRAGRAALQLKLRRRLTELLGAQDGALLVRLLQALRVARMPGLERSVRERFLPVH!!Conjugation||Unconjugated!!Family||TNF Receptor
⇄⧉products_description => string (204) "DCR3 antibody is an unconjugated rabbit polyclonal antibody to DCR3 (TNFRSF6...
$value['products_description']
DCR3 antibody is an unconjugated rabbit polyclonal antibody to DCR3 (TNFRSF6B) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
IHC: 1:50-1:200<br>IHC-P: 1:200<br>WB: 1:500-1:2000<br>The predicted MW is 32kDa, while the observed MW by Western blot was 33kDa.
⇄⧉testing_protocols => string (1070) "IHC (Immunohistchemistry)||TNFRSF6B/DCR3 Antibody-Immunohistochemistry of pa...
$value->a['testing_protocols']
IHC (Immunohistchemistry)||TNFRSF6B/DCR3 Antibody-Immunohistochemistry of paraffin-embedded rat kidney tissue at 1:200 dilution.||AAA21319_IHC6.jpg!!IHC (Immunohistochemistry)||TNFRSF6B/DCR3 Antibody-Immunohistochemistry of paraffin-embedded human kidney cancer tissue at 1:200 dilution.||AAA21319_IHC5.jpg!!IHC (Immunohistochemistry)||TNFRSF6B/DCR3 Antibody-Immunohistochemistry of paraffin-embedded human normal stomach tissue at 1:200 dilution.||AAA21319_IHC4.jpg!!IHC (Immunohistochemistry)||TNFRSF6B/DCR3 Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)||AAA21319_IHC3.jpg!!WB (Western Blot)||TNFRSF6B/DCR3 Antibody-Western blot analysis of extracts of various cells.||AAA21319_WB2.jpg!!WB (Western Blot)||TNFRSF6B/DCR3 Antibody-Western blot analysis of extracts of HT-29 cells, using TNFRSF6B antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection.||AAA21319_WB.jpg
⇄⧉etc_term1 => string (473) "Target||Human TNFRSF6B/DCR3!!Immunogen||Recombinant fusion protein containin...
$value->a['etc_term1']
Target||Human TNFRSF6B/DCR3!!Immunogen||Recombinant fusion protein containing a sequence corresponding to amino acids 35-300 of human TNFRSF6B (NP_003814.1). TYPWRDAETGERLVCAQCPPGTFVQRPCRRDSPTTCGPCPPRHYTQFWNYLERCRYCNVLCGEREEEARACHATHNRACRCRTGFFAHAGFCLEHASCPPGAGVIAPGTPSQNTQCQPCPPGTFSASSSSSEQCQPHRNCTALGLALNVPGSSSHDTLCTSCTGFPLSTRVPGAEECERAVIDFVAFQDISIKRLQRLLQALEAPEGWGPTPRAGRAALQLKLRRRLTELLGAQDGALLVRLLQALRVARMPGLERSVRERFLPVH!!Conjugation||Unconjugated!!Family||TNF Receptor
⇄⧉products_description => string (204) "DCR3 antibody is an unconjugated rabbit polyclonal antibody to DCR3 (TNFRSF6...
$value->a['products_description']
DCR3 antibody is an unconjugated rabbit polyclonal antibody to DCR3 (TNFRSF6B) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
IHC: 1:50-1:200<br>IHC-P: 1:200<br>WB: 1:500-1:2000<br>The predicted MW is 32kDa, while the observed MW by Western blot was 33kDa.
⇄⧉testing_protocols => string (1070) "IHC (Immunohistchemistry)||TNFRSF6B/DCR3 Antibody-Immunohistochemistry of pa...
$value->d['testing_protocols']
IHC (Immunohistchemistry)||TNFRSF6B/DCR3 Antibody-Immunohistochemistry of paraffin-embedded rat kidney tissue at 1:200 dilution.||AAA21319_IHC6.jpg!!IHC (Immunohistochemistry)||TNFRSF6B/DCR3 Antibody-Immunohistochemistry of paraffin-embedded human kidney cancer tissue at 1:200 dilution.||AAA21319_IHC5.jpg!!IHC (Immunohistochemistry)||TNFRSF6B/DCR3 Antibody-Immunohistochemistry of paraffin-embedded human normal stomach tissue at 1:200 dilution.||AAA21319_IHC4.jpg!!IHC (Immunohistochemistry)||TNFRSF6B/DCR3 Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)||AAA21319_IHC3.jpg!!WB (Western Blot)||TNFRSF6B/DCR3 Antibody-Western blot analysis of extracts of various cells.||AAA21319_WB2.jpg!!WB (Western Blot)||TNFRSF6B/DCR3 Antibody-Western blot analysis of extracts of HT-29 cells, using TNFRSF6B antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection.||AAA21319_WB.jpg
⇄⧉etc_term1 => string (473) "Target||Human TNFRSF6B/DCR3!!Immunogen||Recombinant fusion protein containin...
$value->d['etc_term1']
Target||Human TNFRSF6B/DCR3!!Immunogen||Recombinant fusion protein containing a sequence corresponding to amino acids 35-300 of human TNFRSF6B (NP_003814.1). TYPWRDAETGERLVCAQCPPGTFVQRPCRRDSPTTCGPCPPRHYTQFWNYLERCRYCNVLCGEREEEARACHATHNRACRCRTGFFAHAGFCLEHASCPPGAGVIAPGTPSQNTQCQPCPPGTFSASSSSSEQCQPHRNCTALGLALNVPGSSSHDTLCTSCTGFPLSTRVPGAEECERAVIDFVAFQDISIKRLQRLLQALEAPEGWGPTPRAGRAALQLKLRRRLTELLGAQDGALLVRLLQALRVARMPGLERSVRERFLPVH!!Conjugation||Unconjugated!!Family||TNF Receptor
⇄⧉products_description => string (204) "DCR3 antibody is an unconjugated rabbit polyclonal antibody to DCR3 (TNFRSF6...
$value->d['products_description']
DCR3 antibody is an unconjugated rabbit polyclonal antibody to DCR3 (TNFRSF6B) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
⇄⧉specificity => string (193) "This assay has high sensitivity and excellent specificity for detection of r...
$value[0]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of rat VEGFR-1/Flt1. No significant cross-reactivity or interference between rat VEGFR-1/Flt1 and analogues was observed.
⇄purity => string (3) "N/A"
$value[0]['_source']['purity']
⇄form => string (3) "N/A"
$value[0]['_source']['form']
⇄concentration => string (3) "N/A"
$value[0]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[0]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[0]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (763) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[0]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGFR-1/Flt1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGFR-1/Flt1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGFR-1/Flt1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGFR-1/Flt1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (626) "aaa15658 rat this assay has high sensitivity and excellent specificity for d...
$value[0]['_source']['search_terms']
aaa15658 rat this assay has high sensitivity and excellent specificity for detection of vegfr 1 flt1 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15658_td elisa kit fms related tyrosine kinase vascular endothelial growth factor permeability receptor cell flt vegfr1 like protein 150,250 da vgfr1_rat 11276093 np_062179.1 p53767 nm_019306.1 samples serum plasma tissue homogenates range 1.56 pg ml 100 <0.39 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in ml100
Background: Vascular endothelial growth factor receptor 1 (VEGFR1), also known as FLT1, is a protein that in humans is encoded by the FLT1 gene, which maps to 13q12. Oncogene FLT belongs to the src gene family and is related to oncogene ROS. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The deduced 1,338-amino acid protein has a calculated molecular mass of 150.6 kD. The sequence structure of the FLT gene resembles that of the FMS gene; hence, Yoshida et al. (1987) proposed the name FLT as an acronym for FMS-like tyrosine kinase.
Pre-Eclampsia||243!!Neoplasms||207!!Hypertension||102!!Proteinuria||57!!Inflammation||46!!Nervous System Diseases||44!!Adenocarcinoma||35!!Necrosis||32!!Brain Diseases||32!!Heart Diseases||29
⇄⧉search_terms => string (480) "aaa13493 human quantitative selisa eia for detection of vegfr1 in serum plas...
$value[1]['_source']['search_terms']
aaa13493 human quantitative selisa eia for detection of vegfr1 in serum plasma body fluids tissue lysates or cell culture supernatants typical testing data standard curve reference only aaa13493_td elisa kit vegf r1 vascular endothelial growth factor receptor 1 fms related tyrosine kinase flt1 vegfr flt like protein permeability 150,250 da vgfr1_rat 11276093 np_062179.1 p53767 nm_019306.1 samples assay type sandwich range 156 pg ml 10,000 sensitivity < 4 receptor1 range156 <4
⇄⧉specificity => string (385) "This assay has high sensitivity and excellent specificity for detection of s...
$value[2]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of sFLTR-1. No significant cross-reactivity or interference between sFLTR-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sFLTR-1 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[2]['_source']['purity']
⇄form => string (3) "N/A"
$value[2]['_source']['form']
⇄concentration => string (3) "N/A"
$value[2]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉etc_term1 => string (157) "Samples||Serum, plasma, cell culture supernatants, body fluid and tissue hom...
$value[2]['_source']['etc_term1']
Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Assay Type||Quantitative Competitive or Sandwich!!Sensitivity||1.0 pg/mL
⇄⧉products_description => string (1422) "Intended Uses: This sFLTR-1 ELISA kit is a 1.5 hour solid-phase ELISA design...
$value[2]['_source']['products_description']
Intended Uses: This sFLTR-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human sFLTR-1. This ELISA kit for research use only, not for therapeutic or test applications!<br><br>Principle of the Assay: sFLTR-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-sFLTR-1 antibody and an sFLTR-1-HRP conjugate. The assay sample and buffer are incubated together with sFLTR-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sFLTR-1 concentration since sFLTR-1 from samples and sFLTR-1-HRP conjugate compete for the anti-sFLTR-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by sFLTR-1 from the sample, fewer sites are left to bind sFLTR-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sFLTR-1 concentration in each sample is interpolated from this standard curve.
⇄⧉search_terms => string (755) "aaa16018 human this assay has high sensitivity and excellent specificity for...
$value[2]['_source']['search_terms']
aaa16018 human this assay has high sensitivity and excellent specificity for detection of sfltr 1 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16018_sc elisa kit soluble fms like tyrosine kinase receptor sflt flt1 flt sflt1 vegfr1 vegfr ai323757 vascular endothelial growth factor embryonic 2 protein permeability 149,876 da emrk2 vgfr1_mouse 2809071 baa24499.1 p35969 o55094 q61517 samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive sandwich 1.0 pg ml sfltr1 embryonic2 sandwich1.0
⇄⧉specificity => string (385) "This assay has high sensitivity and excellent specificity for detection of s...
$value[3]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of sFLTR-1. No significant cross-reactivity or interference between sFLTR-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sFLTR-1 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[3]['_source']['purity']
⇄form => string (3) "N/A"
$value[3]['_source']['form']
⇄concentration => string (3) "N/A"
$value[3]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄⧉products_description => string (1426) "Principle of the Assay: sFLTR-1 ELISA kit applies the competitive enzyme imm...
$value[3]['_source']['products_description']
Principle of the Assay: sFLTR-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-sFLTR-1 antibody and an sFLTR-1-HRP conjugate. The assay sample and buffer are incubated together with sFLTR-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sFLTR-1 concentration since sFLTR-1 from samples and sFLTR-1-HRP conjugate compete for the anti-sFLTR-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by sFLTR-1 from the sample, fewer sites are left to bind sFLTR-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sFLTR-1 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This sFLTR-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat sFLTR-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (747) "aaa16281 rat this assay has high sensitivity and excellent specificity for d...
$value[3]['_source']['search_terms']
aaa16281 rat this assay has high sensitivity and excellent specificity for detection of sfltr 1 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16281_sc elisa kit soluble fms like tyrosine kinase receptor sflt flt1 flt sflt1 vegfr1 vegfr ai323757 vascular endothelial growth factor embryonic 2 protein permeability 149,876 da emrk2 vgfr1_mouse 2809071 baa24499.1 p35969 o55094 q61517 samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 ng ml sfltr1 embryonic2 competitive1.0
⇄⧉products_description => string (826) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
$value[4]['_source']['products_description']
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat sFlt-1 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
⇄⧉search_terms => string (519) "aaa22464 rat no cross reaction with other factors typical testing data stand...
$value[4]['_source']['search_terms']
aaa22464 rat no cross reaction with other factors typical testing data standard curve for reference only aaa22464_sc elisa kit soluble fms like tyrosine kinase 1 sflt related vascular endothelial growth factor permeability receptor flt1 flt1b sflt1 vegfr1 flt1a 53,549 da protein isoform 11a a4qnt7_danre 324499394 ady39752.1 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 10 ng ml 0.156 sensitivity up to 0.05 intra precision <= 8 inter 12 kinase1 range10 <=8 inter12
⇄⧉products_description => string (828) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
$value[5]['_source']['products_description']
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse sFlt-1 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
⇄⧉search_terms => string (523) "aaa22462 mouse no cross reaction with other factors typical testing data sta...
$value[5]['_source']['search_terms']
aaa22462 mouse no cross reaction with other factors typical testing data standard curve for reference only aaa22462_sc elisa kit soluble fms like tyrosine kinase 1 sflt related vascular endothelial growth factor permeability receptor flt1 flt1b sflt1 vegfr1 flt1a 53,549 da protein isoform 11a a4qnt7_danre 324499394 ady39752.1 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 10 ng ml 0.156ng sensitivity up to 0.05 intra precision <= 8 inter 12 kinase1 range10 <=8 inter12
⇄⧉products_description => string (828) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
$value[6]['_source']['products_description']
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human sFlt-1 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
⇄⧉search_terms => string (521) "aaa22444 human no cross reaction with other factors typical testing data sta...
$value[6]['_source']['search_terms']
aaa22444 human no cross reaction with other factors typical testing data standard curve for reference only aaa22444_sc elisa kit soluble fms like tyrosine kinase 1 sflt related vascular endothelial growth factor permeability receptor flt1 flt1b sflt1 vegfr1 flt1a 53,549 da protein isoform 11a a4qnt7_danre 324499394 ady39752.1 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 10 ng ml 0.156 sensitivity up to 0.05 intra precision <= 8 inter 12 kinase1 range10 <=8 inter12
⇄⧉specificity => string (379) "This assay has high sensitivity and excellent specificity for detection of s...
$value[7]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of sFLT1. No significant cross-reactivity or interference between sFLT1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sFLT1 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[7]['_source']['purity']
⇄form => string (3) "N/A"
$value[7]['_source']['form']
⇄concentration => string (3) "N/A"
$value[7]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
Assay Type||Quantitative Competitive!!Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Sensitivity||0.1 ng/mL
⇄⧉etc_term2 => string (202) "Intended Uses||This sFLt-1 ELISA kit is a 1.5 hour solid-phase ELISA designe...
$value[7]['_source']['etc_term2']
Intended Uses||This sFLt-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey sFLt-1. This ELISA kit for research use only, not for therapeutic applications!
⇄⧉products_description => string (1393) "Principle of the Assay: sFLT1 ELISA kit applies the competitive enzyme immun...
$value[7]['_source']['products_description']
Principle of the Assay: sFLT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-sFLT1 antibody and an sFLT1-HRP conjugate. The assay sample and buffer are incubated together with sFLT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sFLT1 concentration since sFLT1 from samples and sFLT1-HRP conjugate compete for the anti-sFLT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by sFLT1 from the sample, fewer sites are left to bind sFLT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sFLT1 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This sFLT1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey sFLT1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (592) "aaa16870 monkey typical testing data standard curve for reference only aaa16...
$value[7]['_source']['search_terms']
aaa16870 monkey typical testing data standard curve for reference only aaa16870_td elisa kit soluble fms like tyrosine 1 sflt1 kinase related vascular endothelial growth factor permeability receptor flt1 flt1b vegfr1 flt1a 53,549 da protein isoform 11a a4qnt7_danre 324499394 ady39752.1 samples serum plasma cell culture supernatants body fluid and tissue homogenate assay type sandwich detection range 2.5 50ng ml sensitivity 0.1ng intended uses this sflt is a 1.5 hour solid phase designed the quantitative determination of research use not therapeutic applications! tyrosine1 range2.5 a1.5
⇄⧉specificity => string (382) "This assay has high sensitivity and excellent specificity for detection of s...
$value[8]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of sFLT-1. No significant cross-reactivity or interference between sFLT-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sFLT-1 and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[8]['_source']['purity']
⇄form => string (3) "N/A"
$value[8]['_source']['form']
⇄concentration => string (3) "N/A"
$value[8]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
Assay Type||Quantitative Competitive or Sandwich!!Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Sensitivity||1.0 pg/mL
⇄⧉products_description => string (1521) "Principle of the Assay: sFLT-1 ELISA kit applies the quantitative sandwich e...
$value[8]['_source']['products_description']
Principle of the Assay: sFLT-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for sFLT-1. Standards or samples are then added to the microtiter plate wells and sFLT-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of sFLT-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for sFLT-1 are added to each well to "sandwich" the sFLT-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain sFLT-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sFLT-1 concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This sFLT-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat sFLT-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (705) "aaa16597 rat this assay has high sensitivity and excellent specificity for d...
$value[8]['_source']['search_terms']
aaa16597 rat this assay has high sensitivity and excellent specificity for detection of sflt 1 no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16597_sc elisa kit soluble fms like tyrosine sflt1 kinase related vascular endothelial growth factor permeability receptor flt1 flt1b vegfr1 flt1a 53,549 da protein isoform 11a a4qnt7_danre 324499394 ady39752.1 samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive sandwich 0.1 ng ml sandwich0.1
⇄⧉testing_protocols => string (1511) "FCM (Flow Cytometry)||Flow cytometric analysis of A431 cells with VEGF Recep...
$value[9]['_source']['testing_protocols']
FCM (Flow Cytometry)||Flow cytometric analysis of A431 cells with VEGF Receptor 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.||AAA30023_FCM7.jpg!!ICC (Immunocytochemistry)||ICC staining VEGF Receptor 1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30023_ICC6.jpg!!ICC (Immunocytochemistry)||ICC staining VEGF Receptor 1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30023_ICC5.jpg!!ICC (Immunocytochemistry)||ICC staining VEGF Receptor 1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.||AAA30023_ICC4.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VEGF Receptor 1 antibody. Counter stained with hematoxylin.||AAA30023_IHC3.jpg!!IHC (Immunohistochemistry)||Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-VEGF Receptor 1 antibody. Counter stained with hematoxylin.||AAA30023_IHC2.jpg!!WB (Western Blot)||Western blot analysis of VEGF Receptor 1 on mouse lung lysates using anti-VEGF Receptor 1 antibody at 1/1, 000 dilution.||AAA30023_WB.jpg
Three cell membrane receptor tyrosine kinases, Flt (also designated VEGF-R1), Flk-1 (also designated VEGF-R2) and Flt-4, putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulin-like sequences in their extracellular domain. These receptors exhibit high degrees of sequence relatedness to each other as well as lesser degrees of relatedness to the class III receptors including CSF-1/Fms, PDGR, SLFR/Kit and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGFs). On the basis of structural similarity to Flt and Flk-1, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand.
Pre-Eclampsia||232!!Neoplasms||202!!Hypertension||93!!Proteinuria||55!!Inflammation||42!!Nervous System Diseases||41!!Adenocarcinoma||35!!Necrosis||31!!Brain Diseases||31!!Neoplasms, Experimental||28
⇄products_categories => string (16) "Total protein Ab"
⇄⧉etc_term1 => string (214) "Samples||Human Serum, Plasma Or Cell Culture Supernatant And Organizations I...
$value[10]['_source']['etc_term1']
Samples||Human Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Stxbp5L Concentration!!Assay Type||Sandwich!!Detection Range||20 ng/ml-0.312 ng/ml!!Sensitivity||0.06 ng/ml.
⇄⧉products_description => string (678) "Principle of the Assay: This experiment use double-sandwich elisa technique ...
$value[10]['_source']['products_description']
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human STXBP5L monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
⇄⧉search_terms => string (626) "aaa12621 human no cross reaction with other factors typical testing data sta...
$value[10]['_source']['search_terms']
aaa12621 human no cross reaction with other factors typical testing data standard curve for reference only aaa12621_sc elisa kit soluble vascular endothelial growth factor receptor 2 svegfr kinase insert domain protein kdr flk1 ly73 flk 1 krd vegfr2 vegfr 6130401c07 nyk vegf fetal liver tyrosine 3 75,230 da cd_antigen cd309 vgfr2_mouse 215400616 acj66293.1 p35918 c5h7s5 samples serum plasma or cell culture supernatant and organizations in the natural recombinant stxbp5l concentration assay type sandwich detection range 20 ng ml 0.312 sensitivity 0.06 intra precision <= 8 inter 12 receptor2 tyrosine3 range20 <=8 inter12
⇄⧉products_description => string (679) "Principle of the Assay: This experiment use double-sandwich elisa technique ...
$value[11]['_source']['products_description']
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse sVEGFR-2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
⇄⧉search_terms => string (578) "aaa12818 mouse no cross reaction with other factors typical testing data sta...
$value[11]['_source']['search_terms']
aaa12818 mouse no cross reaction with other factors typical testing data standard curve for reference only aaa12818_sc elisa kit soluble vascular endothelial growth factor receptor 2 svegfr kinase insert domain protein kdr flk1 ly73 flk 1 krd vegfr2 vegfr 6130401c07 nyk vegf fetal liver tyrosine 3 75,230 da cd_antigen cd309 vgfr2_mouse 215400616 acj66293.1 p35918 c5h7s5 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 20 ng ml 0.312 sensitivity up to 0.06 intra precision <= 8 inter 12 receptor2 tyrosine3 range20 <=8 inter12
⇄⧉specificity => string (171) "This assay has high sensitivity and excellent specificity for detection of F...
$value[12]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of FGFR1. No significant cross-reactivity or interference between FGFR1 and analogues was observed.
⇄purity => string (3) "N/A"
$value[12]['_source']['purity']
⇄form => string (3) "N/A"
$value[12]['_source']['form']
⇄concentration => string (3) "N/A"
$value[12]['_source']['concentration']
⇄⧉storage_stability => string (156) "Store entire kit at 2-8C for short-term. For longer-term, please store the m...
$value[12]['_source']['storage_stability']
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
⇄⧉products_description => string (825) "Principle of the Assay: This kit was based on sandwich enzyme-linked immune-...
$value[12]['_source']['products_description']
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
⇄products_references => string (3) "N/A"
$value[12]['_source']['products_references']
⇄⧉products_related_diseases => string (290) "Nervous System Diseases||155!!Disease Models, Animal||148!!Myeloproliferativ...
⇄⧉search_terms => string (721) "aaa17568 human this assay has high sensitivity and excellent specificity for...
$value[12]['_source']['search_terms']
aaa17568 human this assay has high sensitivity and excellent specificity for detection of fgfr1 no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa17568_sc elisa kit fibroblast growth factor receptor 1 cd331 flt 2 bfgfr bfgf r cek fgfbr kal kal2 n sam ogd fgfr flgh3 flt2h4 h2 h5 hbgfr basic antigen fms like tyrosine kinase related heparin binding hydroxyaryl protein proto oncogene c fgr soluble variant 91,577 da cek1 fgfr1_chick 45382885 np_990841.1 p21804 nm_205510.1 samples serum plasma tissue homogenates other biological fluids type quantitative sandwich range 0.156 10ng ml 0.094ng intra precision cv<8 inter cv<10 receptor1 flt2
⇄⧉specificity => string (81) "Purified His-tagged VEGFR3 protein was used to produced this monoclonal anti...
$value[13]['_source']['specificity']
Purified His-tagged VEGFR3 protein was used to produced this monoclonal antibody.
⇄purity => string (3) "N/A"
$value[13]['_source']['purity']
⇄⧉form => string (163) "Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. ...
$value[13]['_source']['form']
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
⇄concentration => string (3) "N/A"
$value[13]['_source']['concentration']
⇄⧉storage_stability => string (149) "Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term stora...
$value[13]['_source']['storage_stability']
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
⇄⧉testing_protocols => string (1731) "IHC (Immunohistchemistry-Paraffin)||Immunohistochemical analysis of paraffin...
$value[13]['_source']['testing_protocols']
IHC (Immunohistchemistry-Paraffin)||Immunohistochemical analysis of paraffin-embedded R. kidney section using VEGFR3(Cat#NA). NA was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.||AAA28806_IHC6.jpg!!IHC (Immunohistochemistry-Paraffin)||Immunohistochemical analysis of paraffin-embedded H. kidney section using VEGFR3(Cat#). was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.||AAA28806_IHC5.jpg!!IHC (Immunohistochemistry-Paraffin)||Immunohistochemical analysis of paraffin-embedded H. testis section using VEGFR3(Cat#). was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.||AAA28806_IHC4.jpg!!FCM (Flow Cytometry)||Flow cytometric analysis of HUVEC cells using VEGFR3(green) compared to an isotype control of mouse IgG2a(blue). It was used as the secondary antibody.||AAA28806_FCM3.jpg!!WB (Western Blot)||All lanes : Anti-VEGFR3 at 1:2000 dilution<br>Lane 1: A549 whole cell lysate<br>Lane 2: 293 whole cell lysate<br><br><br>Lysates/proteins at 20 ug per lane. <br><br>Secondary<br>Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. <br><br>Predicted band size : 153 kDa<br><br>Blocking/Dilution buffer: 5% NFDM/TBST.||AAA28806_WB2.jpg!!WB (Western Blot)||Western blot analysis of lysates from A549,T47D cell line (from left to right), using VEGFR3 Antibody. It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.||AAA28806_WB.jpg
⇄⧉etc_term1 => string (454) "Cellular Location||Cell membrane; Single-pass type I membrane protein Cytopl...
$value[13]['_source']['etc_term1']
Cellular Location||Cell membrane; Single-pass type I membrane protein Cytoplasm Nucleus. Note=Ligand-mediated autophosphorylation leads to rapid internalization [Isoform 2]: Cell membrane; Single-pass type I membrane protein.!!Tissue Location||Detected in endothelial cells (at protein level). Widely expressed. Detected in fetal spleen, lung and brain. Detected in adult liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney.
⇄⧉products_description => string (1362) "Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and V...
$value[13]['_source']['products_description']
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades; isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'.
⇄⧉products_references => string (252) "Irrthum A., et al. Am. J. Hum. Genet. 67:295-301(2000). Pajusola K., et al. ...
$value[13]['_source']['products_references']
Irrthum A., et al. Am. J. Hum. Genet. 67:295-301(2000). Pajusola K., et al. Cancer Res. 52:5738-5743(1992). Pajusola K., et al. Cancer Res. 53:3845-3845(1993). Galland F., et al. Genomics 13:475-478(1992). Galland F., et al. Oncogene 8:1233-1240(1993).
⇄⧉search_terms => string (1096) "aaa28806 mouse human monoclonal igg2a purified antibody supplied in pbs with...
$value[13]['_source']['search_terms']
aaa28806 mouse human monoclonal igg2a purified antibody supplied in pbs with 0.09 w v sodium azide this is through a protein g column followed by dialysis against his tagged vegfr3 was used to produced western blot wb immunohistochemistry ihc paraffin flow cytometry fc facs elisa eia 1:2000 1:25 p analysis of lysates from a549,t47d cell line left right using it diluted at 1:1000 each lane goat anti igg h&l hrp 1:10000 dilution as the secondary aaa28806_wb all lanes 1 a549 whole lysate 2 293 proteins 20 ug per h+l peroxidase conjugated 10000 predicted band size 153 kda blocking buffer 5 nfdm tbst aaa28806_wb2 cytometric huvec cells green compared an isotype control blue aaa28806_fc3 immunohistochemical embedded h testis section cat# 1:400 dab staining aaa28806_ihc4 kidney aaa28806_ihc5 r cat#na na aaa28806_ihc6 flt4 vascular endothelial growth factor receptor 3 fms like tyrosine kinase 4 isoform related pcl flt flt41 lmph1a lmphm1 vegfr 152757 vgfr3_human 104294888 np_891555.2 p35916 136352 antigen source type recombinant lanes1 lysate2 proteins20 size153 buffer5 receptor3 kinase4
⇄⧉products_description => string (1104) "Intended Uses: This immunoassay kit allows for the in vitro quantitative det...
$value[14]['_source']['products_description']
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.<br><br>Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Type||Quantitative Sandwich!!Samples||Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids!!Detection Range||0.05-30ng/ml!!Sensitivity||0.024ng/ml
⇄⧉etc_term2 => string (403) "Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Thr...
$value[15]['_source']['etc_term2']
Intra-assay Precision||Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%!!Inter-assay Precision||Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
⇄⧉products_description => string (926) "Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (EL...
$value[15]['_source']['products_description']
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat SFLT-1 antibody. SFLT-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat SFLT-1 Antibody is added and binds to SFLT-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SFLT-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat SFLT-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.<br><br>Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Soluble Vascular Endothelial Growth Factor Receptor 1 (also known as SFLT-1) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
⇄⧉search_terms => string (472) "aaa18919 rat typical testing data standard curve for reference only aaa18919...
$value[15]['_source']['search_terms']
aaa18919 rat typical testing data standard curve for reference only aaa18919_sc elisa kit vascular endothelial growth factor receptor 1 sflt soluble samples serum plasma cell culture supernates lysates tissue homogenates assay type quantitative sandwich detection range 0.05ng ml 30ng sensitivity 0.024ng intra precision within an three of known concentration were tested on one plate to assess cv<8 inter between assays in separate cv = sd mean x 100 cv<10 receptor1 x100
⇄⧉specificity => string (177) "This assay has high sensitivity and excellent specificity for detection of p...
$value[16]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of pig VEGF. No significant cross-reactivity or interference between pig VEGF and analogues was observed.
⇄purity => string (3) "N/A"
$value[16]['_source']['purity']
⇄form => string (3) "N/A"
$value[16]['_source']['form']
⇄concentration => string (3) "N/A"
$value[16]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[16]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[16]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (731) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[16]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (646) "aaa15558 pig this assay has high sensitivity and excellent specificity for d...
$value[16]['_source']['search_terms']
aaa15558 pig this assay has high sensitivity and excellent specificity for detection of vegf no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15558_td elisa kit vascular endothelial growth factor a cell rp1 261g23.1 mgc70609 mvcd1 vpf isoform vegf165 permeability vegfa 22,368 da vegfa_pig 47522980 np_999249.1 p49151 nm_214084.1 q9gl52 samples serum plasma tissue homogenates type quantitative sandwich range 4.68 pg ml 300 <1.17 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in ml300
<b>Storage:</b><br>Avoid repeated freeze/thaw cycles.<br>Store at 4 degree C for frequent use.<br>Aliquot and store at -20 degree C for 24 months.<br><br><b>Stability Test:</b><br>The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western blotting: 0.01-2ug/mL;<br>Immunohistochemistry: 5-20ug/mL;<br>Immunocytochemistry: 5-20ug/mL;<br>Optimal working dilutions must be determined by end user.
aaa20930 pig polyclonal rabbit antigen specific affinity chromatography followed by protein a supplied as solution form in pbs ph7.4 containing 0.02 nan3 50 glycerol western blot wb immunohistochemistry ihc immunocytochemistry icc immunoprecipitation ip blotting 0.5 2ug ml 5 20ug optimal working dilutions must be determined end user sample rat placenta lysate primary ab 1ug anti porcine vegfr2 antibody second 0.2ug hrp linked caprine igg catalog mbs2086047 aaa20930_wb cerebrum aaa20930_wb2 figure recombinant aaa20930_wb3 vascular endothelial growth factor receptor 2 to tyrosine kinase kdr organism species sus scrofa source preparation cross reactivity traits liquid immunogen phe8~val290 expressed e.coli mbs2030656 nan350 blotting0.5 ml5 receptor2
⇄⧉specificity => string (177) "This assay has high sensitivity and excellent specificity for detection of r...
$value[18]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of rat VEGF. No significant cross-reactivity or interference between rat VEGF and analogues was observed.
⇄purity => string (3) "N/A"
$value[18]['_source']['purity']
⇄form => string (3) "N/A"
$value[18]['_source']['form']
⇄concentration => string (3) "N/A"
$value[18]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[18]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[18]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
⇄⧉products_description => string (731) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[18]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄⧉search_terms => string (707) "aaa15479 rat this assay has high sensitivity and excellent specificity for d...
$value[18]['_source']['search_terms']
aaa15479 rat this assay has high sensitivity and excellent specificity for detection of vegf no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15479_td elisa kit vascular endothelial growth factor a cell rp1 261g23.1 mgc70609 mvcd1 vpf isoform vegf165 permeability vegfa 3 vegf164 25,239 da vegfa_rat 160358797 np_001103804.1 p16612 nm_001110334.2 q541s6 q91ze1 q9jkx7 q9qxg6 q9qxg7 samples serum plasma tissue homogenates type quantitative sandwich range 3.9 pg ml 250 < 0.97 intra precision within an cv <8 three known concentration were tested twenty times on one plate to assess inter assays <10 in vegfa3 range3.9 ml250
⇄⧉specificity => string (376) "This assay has high sensitivity and excellent specificity for detection of V...
$value[19]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of VEGF. No significant cross-reactivity or interference between VEGF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VEGF and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[19]['_source']['purity']
⇄form => string (3) "N/A"
$value[19]['_source']['form']
⇄concentration => string (3) "N/A"
$value[19]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
Assay Type||Quantitative Competitive or Sandwich!!Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Sensitivity||1.0 pg/mL
⇄⧉products_description => string (1501) "Principle of the Assay: VEGF ELISA kit applies the quantitative sandwich enz...
$value[19]['_source']['products_description']
Principle of the Assay: VEGF ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for VEGF. Standards or samples are then added to the microtiter plate wells and VEGF if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of VEGF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for VEGF are added to each well to "sandwich" the VEGF immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain VEGF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VEGF concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This VEGF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat VEGF. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄⧉search_terms => string (707) "aaa16218 rat this assay has high sensitivity and excellent specificity for d...
$value[19]['_source']['search_terms']
aaa16218 rat this assay has high sensitivity and excellent specificity for detection of vegf no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16218_sc elisa kit vascular endothelial growth factor a vegfa vpf mvcd1 permeability 34,406 da vegfa_human 181971 aaa35789.1 p15692 o60720 o75875 q074z4 q16889 b5bu86 h0y2s8 h0y407 h0y414 h0y462 h0y8n2 h3blw7 gene 603933 cancer samples serum plasma cell culture supernatants body fluid tissue homogenate type competitive sandwich 1.0 pg ml sandwich1.0
