Mouse anti-Mouse, Rat Kir2.1 Monoclonal Antibody | anti-Kcnj2 antibody

Kir2.1 Antibody: PerCP

Cat. Num. AAA802921

• Gene Names: Kcnj2; IRK1; Kcnf1; Kir2.1
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Synonyms: Kir2.1; Monoclonal Antibody; Kir2.1 Antibody: PerCP; HHBIRK1; HHIRK1; HIRK 1; IRK1; KCNJ2; LQT7; SQT3; potassium inwardly rectifying channel J2; anti-Kcnj2 antibody

• Host: Mouse
• Reactivity: Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S112B-14
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 428

• Applicable Applications for anti-Kcnj2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)
• Conjugate: PerCP
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112B-14. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear expression in the epidermis and hair follicles.)

Western Blot (WB)

(Western Blot analysis of Monkey COS transient cell lysate showing detection of Kir2.1 Potassium Channel protein using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112B-14. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112B-14. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.)

Related Product Information for anti-Kcnj2 antibody

Background Info: Detects ~55kDa. No cross-reactivity against Kir2.2 or Kir2.3

Scientific Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). The Kir2.1 inward-rectifier potassium ion channel is encoded by the KCNJ2 gene. A defect in this gene is associated with Andersen-Tawil syndrome (3).

Product Categories/Family for anti-Kcnj2 antibody

Ion Channels; Neuroscience

References

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Donaldson M.R., Yoon G., Fu Y.H., Ptacek L.J. (2004). Ann. Med. 36 Suppl 1: 92-7.

NCBI and Uniprot Product Information

• NCBI GI #: 6680530
• NCBI GeneID: 16518
• NCBI Accession #: NP_032451
• NCBI GenBank Nucleotide #: NM_008425.4
• UniProt Accession #: P35561
• Molecular Weight: 48,389 Da
• NCBI Official Full Name: inward rectifier potassium channel 2
• NCBI Official Synonym Full Names: potassium inwardly-rectifying channel, subfamily J, member 2
• NCBI Official Symbol: Kcnj2
• NCBI Official Synonym Symbols: IRK1; Kcnf1; Kir2.1
• NCBI Protein Information: inward rectifier potassium channel 2; IRK-1; inward rectifier K(+) channel Kir2.1; potassium channel, inwardly rectifying subfamily J member 2
• UniProt Protein Name: Inward rectifier potassium channel 2
• Protein Family: Inward rectifier potassium channel
• UniProt Gene Name: Kcnj2
• UniProt Synonym Gene Names: Irk1; IRK-1
• UniProt Entry Name: KCNJ2_MOUSE

Uniprot Description

KCNJ2: Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium or cesium. Defects in KCNJ2 are the cause of long QT syndrome type 7 (LQT7); also called Andersen syndrome or Andersen cardiodysrhythmic periodic paralysis. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. LQT7 manifests itself as a clinical triad consisting of potassium-sensitive periodic paralysis, ventricular ectopy and dysmorphic features. Defects in KCNJ2 are the cause of short QT syndrome type 3 (SQT3). Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death. SQT3 has a unique ECG phenotype characterized by asymmetrical T waves. Defects in KCNJ2 are the cause of familial atrial fibrillation type 9 (ATFB9). ATFB9 is a familial form of atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ2 subfamily.

Protein type: Membrane protein, multi-pass; Channel, potassium; Membrane protein, integral

Cellular Component: Golgi apparatus; voltage-gated potassium channel complex; smooth endoplasmic reticulum; rough endoplasmic reticulum; cell soma; membrane; integral to plasma membrane; dendrite; T-tubule; dendritic spine; integral to membrane; plasma membrane; intrinsic to membrane

Molecular Function: identical protein binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; voltage-gated ion channel activity; inward rectifier potassium channel activity

Biological Process: regulation of skeletal muscle contraction via membrane action potential; potassium ion import; transport; ion transport; potassium ion transport; protein homotetramerization; magnesium ion transport