Mouse anti-Human Kir, Low Endotoxin Monoclonal Antibody | anti-KIR3DL1 antibody

Kir, Low Endotoxin (KIR2D, Killer Cell Immunoglobulin (Ig)-like Receptor, CD158a, CD158b, P50.3)

Cat. Num. AAA600318

• Gene Names: KIR3DL1; KIR; NKB1; NKAT3; NKB1B; CD158E1; KIR3DL1/S1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Kir; Low Endotoxin; Monoclonal Antibody; Low Endotoxin (KIR2D; Killer Cell Immunoglobulin (Ig)-like Receptor; CD158a; CD158b; P50.3); Anti -Kir; anti-KIR3DL1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4j52
• Specificity: Recognizes KIR2D members of the killer cell immunoglobulin (Ig)-like receptor (KIR) family: CD158a, CD158b and P50.3. Specifically recognizes the long and short forms CD158a and CD158b (KIR2DL, KIR2DS1 and KIR2DS2 respectively) and also p50.3 (KIR2DS4). Reported to have functional activity, activating NK cell cytotoxicity via KIR2DS and inhibiting via KIR2DL forms.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added.

• Applicable Applications for anti-KIR3DL1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry, ELISA. Immunoprecipitation, Western Blot. and Functional Assays.; Dilution: Flow Cytometry: 1:50-1:100. Use 10ul of the optimal working dilution to label 10e6 cells in 100ul.; Optimal dilutions to be determined by researcher.
• Immunogen: Human natural killer (NK) cell lysate
• Recommended Negative Controls: I1904-78M: IgG1 Negative Control; I1904-78Z: IgG1 Murine Negative Control (For rat tissue) (Azide Free)
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-KIR3DL1 antibody

KIR2D family members are cell surface glycoproteins with two Ig domains, which are expressed on natural killer cells and some T cells.

Product Categories/Family for anti-KIR3DL1 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 134268644
• NCBI GeneID: 3811
• NCBI Accession #: NP_037421.2
• NCBI GenBank Nucleotide #: NM_013289.2
• UniProt Accession #: P43629; O43473; Q14946; Q16541
• Molecular Weight: 49,098 Da
• NCBI Official Full Name: killer cell immunoglobulin-like receptor 3DL1
• NCBI Official Synonym Full Names: killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1
• NCBI Official Symbol: KIR3DL1
• NCBI Official Synonym Symbols: KIR; NKB1; NKAT3; NKB1B; CD158E1; KIR3DL1/S1
• NCBI Protein Information: killer cell immunoglobulin-like receptor 3DL1; NKAT-3; NK-receptor; KIR antigen 3DL1; killer Ig receptor; p70 NK receptor CL-2/CL-11; MHC class I NK cell receptor; CD158 antigen-like family member E; p70 killer cell inhibitory receptor; natural killer-associated transcript 3; HLA-BW4-specific inhibitory NK cell receptor; p70 natural killer cell receptor clones CL-2/CL-11
• UniProt Protein Name: Killer cell immunoglobulin-like receptor 3DL1
• Protein Family: Killer cell immunoglobulin-like receptor
• UniProt Gene Name: KIR3DL1
• UniProt Synonym Gene Names: CD158E; NKAT3; NKB1; NKAT-3
• UniProt Entry Name: KI3L1_HUMAN

NCBI Description

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. [provided by RefSeq, Jul 2008]

Uniprot Description

KIR3DL1: Receptor on natural killer (NK) cells for HLA Bw4 allele. Inhibits the activity of NK cells thus preventing cell lysis. Belongs to the immunoglobulin superfamily.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: HLA-B specific inhibitory MHC class I receptor activity

Biological Process: regulation of immune response; immune response; signal transduction

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To