Mouse anti-Human KHSRP / KSRP Monoclonal Antibody | anti-KHSRP antibody

Mouse Monoclonal [clone 4C10] (IgG2b,k) to Human KHSRP / KSRP

Cat. Num. AAA246006

• Gene Names: KHSRP; FBP2; KSRP; FUBP2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified from ascites by Protein A
• Synonyms: KHSRP / KSRP; Monoclonal Antibody; Mouse Monoclonal [clone 4C10] (IgG2b; k) to Human KHSRP / KSRP; KHSRP Antibody; FUSE binding protein 2 Antibody; KSRP Antibody; p75 Antibody; FUBP2 Antibody; FUSE-binding protein 2 Antibod; anti-KHSRP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 4C10
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4
• Concentration: 0.4 mg/ml (varies by lot)
• Sequence Length: 711

• Applicable Applications for anti-KHSRP antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Sandwich ELISA (Recombinant protein).
• Target: Human KHSRP / FBP2
• Target Species: Human
• Immunogen Description: KHSRP (NP_003676, 151 a.a. ~ 240 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Antigen Type: Recombinant protein
• Immunogen: KHSRP / FBP2 antibody was raised against kHSRP (NP_003676, 151 a.a. ~ 240 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-KHSRP antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-KHSRP antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

ELISA (EIA)

(Detection limit for recombinant GST tagged KHSRP is approximately 0.3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 154355000
• NCBI GeneID: 8570
• NCBI Accession #: NP_003676.2
• NCBI GenBank Nucleotide #: NM_003685.2
• UniProt Accession #: Q92945; O00301; Q59EZ9; Q5U4P6; Q9UNT5; Q9UQH5
• Molecular Weight: 73,115 Da
• NCBI Official Full Name: far upstream element-binding protein 2
• NCBI Official Synonym Full Names: KH-type splicing regulatory protein
• NCBI Official Symbol: KHSRP
• NCBI Official Synonym Symbols: FBP2; KSRP; FUBP2
• NCBI Protein Information: far upstream element-binding protein 2; p75; FUSE binding protein 2; FUSE-binding protein 2; KH type-splicing regulatory protein
• UniProt Protein Name: Far upstream element-binding protein 2
• Protein Family: Far upstream element-binding protein
• UniProt Gene Name: KHSRP
• UniProt Synonym Gene Names: FUBP2; FUSE-binding protein 2; KSRP
• UniProt Entry Name: FUBP2_HUMAN

NCBI Description

The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes, including transcription, alternative pre-mRNA splicing, and mRNA localization (Min et al., 1997 [PubMed 9136930]; Gherzi et al., 2004 [PubMed 15175153]).[supplied by OMIM, Apr 2010]

Uniprot Description

KHSRP: an RNA binding and processing protein that binds to the dendritic targeting element and may play a role in mRNA trafficking. Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3' untranslated regions, possibly by recruiting degradation machinery to ARE-containing mRNAs. Interacts with APC which is part of the apolipoprotein B mRNA editing enzyme complex. The interaction is not required for activity of the catalytic component, APOBEC1, but may confer stability on the complex.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; membrane; stress granule; cytosol

Molecular Function: mRNA binding; DNA binding

Biological Process: RNA splicing, via transesterification reactions; mRNA transport; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA splicing; mRNA catabolic process; gene expression; mRNA processing