Mouse anti-Human JUP Monoclonal Antibody | anti-JUP antibody

JUP Antibody

Cat. Num. AAA9602747

• Gene Names: JUP; DP3; PDGB; PKGB; CTNNG; DPIII
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: Affinity-Chromatography
• Synonyms: JUP; Monoclonal Antibody; JUP Antibody; ARVD 12; ARVD12; Catenin (cadherin associated protein); gamma 80kDa; catenin (cadherin-associated protein) gamma (80kD); Catenin gamma; CTNNG; Desmoplakin 3; Desmoplakin III; Desmoplakin-3; Desmoplakin3; DesmoplakinIII; DP 3; DP III; DP3; DPIII; gamma catenin; Junction plakoglobin; OTTHUMP00000164732; OTTHUMP00000164735; OTTHUMP00000164738; PDGB; PKGB; PLAK_HUMAN; PLAKOGLOBIN; anti-JUP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: JUP antibody detects endogenous levels of total JUP
• Purity/Purification: Affinity-Chromatography
• Form/Format: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 745

• Applicable Applications for anti-JUP antibody: Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: ELISA: 1:10000; WB: 1:500-1:2000; IHC: 1:200-1:1000; ICC: 1:200-1:1000
• Immunogen: Purified recombinant fragment of human JUP expressed in E. Coli
• Subcellular Location: Cell Junction > Adherens Junction. Cell Junction > Desmosome. Cytoplasm > Cytoskeleton. Membrane. Cytoplasmic in a soluble and membrane-associated form.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis using JUP mouse mAb against T47D (1), MCF-7 (2), SKBR-3 (3), A431 (4) and HEK293 (5) cell lysate.)

Related Product Information for anti-JUP antibody

Description: This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described.
Function: Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity).
Subunit Structure: Homodimer. Component of an E-cadherin/catenin adhesion complex composed of at least E-cadherin/CDH1 and gamma-catenin/JUP, and possibly alpha-catenin/CTNNA1; the complex is located to adherens junctions. The stable association of CTNNA1 is controversial as CTNNA1 was shown not to bind to F-actin when assembled in the complex. Interacts with MUC1. Interacts with CAV1 (By similarity). Interacts with PTPRJ. Interacts with DSG1. Interacts with DSC1 and DSC2. Interacts with PKP2.
Post-translational Modifications: May be phosphorylated by FER.
Similarity: The entire ARM repeats region mediates binding to CDH1/E-cadherin. The N-terminus and first three ARM repeats are sufficient for binding to DSG1. The N-terminus and first ARM repeat are sufficient for association with CTNNA1. DSC1 association requires both ends of the ARM repeat region. Belongs to the beta-catenin family.

NCBI and Uniprot Product Information

• NCBI GI #: 4504811
• NCBI GeneID: 3728
• NCBI Accession #: NP_002221.1
• NCBI GenBank Nucleotide #: NM_002230.3
• UniProt Accession #: P14923; Q15093; Q15151; Q7L3S5; Q86W21; Q9BWC4; Q9HCX9
• Molecular Weight: Observed: 82 kDa; Predicted: 82 kDa
• NCBI Official Full Name: junction plakoglobin
• NCBI Official Synonym Full Names: junction plakoglobin
• NCBI Official Symbol: JUP
• NCBI Official Synonym Symbols: DP3; PDGB; PKGB; CTNNG; DPIII
• NCBI Protein Information: junction plakoglobin
• UniProt Protein Name: Junction plakoglobin
• Protein Family: Junction plakoglobin
• UniProt Gene Name: JUP
• UniProt Synonym Gene Names: CTNNG; DP3

NCBI Description

This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described. [provided by RefSeq, Jul 2008]

Uniprot Description

Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton ().