Mouse anti-Human JNK1 Monoclonal Antibody | anti-JNK1 antibody

Anti-JNK1 Mouse mAb

Cat. Num. AAA476306

• Gene Names: MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
• Reactivity: Human
• Applications: Western Blot
• Synonyms: JNK1; Monoclonal Antibody; Anti-JNK1 Mouse mAb; AI849689; c Jun N terminal kinase 1; C-JUN kinase 1; c-Jun N-terminal kinase 1; EC 2.7.11.24; JNK 1; JNK; JNK-46; JNK1A2; JNK21B1/2; MAP kinase 8; MAPK 8; mapk8; Mitogen activated protein kinase 8; Mitogen-activated protein kinase 8; MK08_HUMAN; p54 gamma; Prkm8; Protein kinase JNK1; Protein kinase; mitogen-activated; 8; SAPK 1; SAPK gamma; SAPK1; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1; anti-JNK1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1H8-E10-D2
• Form/Format: Purified antibody in PBS with 0.05% sodium azide.
• Sequence Length: 427

• Applicable Applications for anti-JNK1 antibody: Western Blot (WB)
• Immunogen: Purified recombinant fragment of human MAPK8 expressed in E Coli.
• Sequence Similarities: Contains 1 protein kinase domain.
• Subunit Structure: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP. Forms a complex with MAPK8IP1 and ARHGEF28 (By similarity). Interacts (phosphorylated form) with NFE2; the interaction phosphorylates NFE2 in undifferentiated cells (By similarity). Interacts with NFATC4. Interacts with MECOM; regulates JNK signaling. Interacts with PIN1; this interaction mediates MAPK8 conformational changes leading to the binding of MAPK8 to its substrates.
• Subcellular Location: Nucleus

Western Blot (WB)

(Western blot analysis of extracts from Raji, HCT116, Jurkat, MCF7 and A549 cell lysates using JNK1 mouse mAb (1:1000 diluted).Predicted band size:48KDa.Observed band size:46, 54KDa.)

Western Blot (WB)

(Western blot analysis using MAPK8 mouse mAb against A431 (1), K562 (2), HeLa (3), NIH3T3 (4), PC-12 (5), and MCF-7 (6) cell lysate.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HeLa cells using MAPK8 mouse mAb (green) and negative control (red).)

Related Product Information for anti-JNK1 antibody

Entrez Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]
UniPort Summary Function: JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

NCBI and Uniprot Product Information

• NCBI GI #: 513788279
• NCBI GeneID: 5599
• NCBI Accession #: NP_001265476.1
• NCBI GenBank Nucleotide #: NM_001278547.1
• UniProt Accession #: P45983; Q15709; Q15712; Q15713; Q308M2; B5BTZ5; B7ZLV4; D3DX88; D3DX92
• Molecular Weight: 46; 54
• NCBI Official Full Name: mitogen-activated protein kinase 8 isoform beta2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8
• NCBI Official Symbol: MAPK8
• NCBI Official Synonym Symbols: JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
• NCBI Protein Information: mitogen-activated protein kinase 8
• UniProt Protein Name: Mitogen-activated protein kinase 8
• Protein Family: JNK1/MAPK8-associated membrane protein
• UniProt Gene Name: MAPK8
• UniProt Synonym Gene Names: JNK1; PRKM8; SAPK1; SAPK1C; MAP kinase 8; MAPK 8; SAPK1c

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]

Uniprot Description

JNK1: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described.

Protein type: CMGC group; EC 2.7.11.24; JNK subfamily; Kinase, protein; MAPK family; MAPK/JNK subfamily; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 10q11.22

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function: enzyme binding; histone deacetylase binding; histone deacetylase regulator activity; JUN kinase activity; kinase activity; protein binding; protein serine/threonine kinase activity

Biological Process: JNK cascade; JUN phosphorylation; negative regulation of apoptosis; negative regulation of protein binding; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of apoptosis; positive regulation of cyclase activity; positive regulation of protein metabolic process; protein amino acid phosphorylation; regulation of circadian rhythm; regulation of macroautophagy; regulation of protein localization; regulation of transcription factor activity; response to stress; response to UV