Mouse JAM-A Monoclonal Antibody | anti-JAM-A antibody
JAM-A, Human, mAb M.Ab.F11
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
- FC: Antibody M.Ab. F11 stains the extracellular domain of JAM-A protein name. Cells were incubated with 5ug/ml of mAb in 0.1% BSA/PBS. As negative control an isotype-matched antibody was used (Ref. 4).
- WB: A non-reduced and reduced sample treatment and SDS-PAGE was used. The band sizes are 32 and 35 kDa (Ref. 1).
- FS: Antibody M.Ab. F11 functions as anagonist resulting in platelet aggregation at concentrations of approximately 5 ug/ml (Ref. 1).
3. Wang, F et al; Stimulatory antibody-induced activation and selective translocation of protein kinase C isoenzymes in human platelets. Biochem J 1995, 311: 401
4. Sobocka, M et al; Cloning of the human platelet F11 receptor: a cell adhesion molecule member of the immunoglobulin superfamily involved in platelet aggregation. Blood 2000, 95: 2600
5. Babinska, A et al; Two regions of the human platelet F11-receptor (F11R) are critical for platelet aggregation, potentiation and adhesion. Thromb Haemost 2002, 87: 712
6. Babinska, A et al; The F11 receptor (F11R/JAM-A) in atherothrombosis: overexpression of F11R in atherosclerotic plaques. Thromb Haemost 2007, 97: 272
7. Cavusoglu, E et al; Association of plasma levels of F11 receptor/junctional adhesion molecule-A (F11R/JAM-A) with human atherosclerosis. JACC 2007, 50: 1768
8. Koenen, R et al; Regulated release and functional modulation of junctional adhesion molecule A by disintegrin metalloproteinases. Blood 2009, 113: 4799
NCBI and Uniprot Product Information
NCBI Description
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008]
Uniprot Description
JAM-A: Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. Belongs to the immunoglobulin superfamily.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q21.2-q21.3
Cellular Component: microtubule cytoskeleton; tight junction; plasma membrane; integral to membrane; cytoplasmic vesicle; intercellular junction; cell junction
Molecular Function: protein binding; PDZ domain binding
Biological Process: intercellular junction assembly and maintenance; extracellular matrix organization and biogenesis; response to radiation; viral reproduction; transforming growth factor beta receptor signaling pathway; actomyosin structure organization and biogenesis; blood coagulation; cell adhesion; inflammatory response; leukocyte migration; positive regulation of blood pressure; intestinal absorption
Research Articles on JAM-A
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Product Notes
The JAM-A f11r (Catalog #AAA584196) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's JAM-A can be used in a range of immunoassay formats including, but not limited to, Yes: Immunohistochemistry - Paraffin (IHC-P), Immunofluorescence (IF), Flow Cytometry (FC), Functional Studies (FS), Immunoassay (IA), Immunoprecipitation (IP), Western Blot (WB); Not Determined: Immunohistochemistry - Frozen (IHC-F) . Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. - FC: Antibody M.Ab. F11 stains the extracellular domain of JAM-A protein name. Cells were incubated with 5ug/ml of mAb in 0.1% BSA/PBS. As negative control an isotype-matched antibody was used (Ref. 4). - WB: A non-reduced and reduced sample treatment and SDS-PAGE was used. The band sizes are 32 and 35 kDa (Ref. 1). - FS: Antibody M.Ab. F11 functions as anagonist resulting in platelet aggregation at concentrations of approximately 5 ug/ml (Ref. 1). Researchers should empirically determine the suitability of the JAM-A f11r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JAM-A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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