Mouse anti-Human IRX5 Monoclonal Antibody | anti-IRX5 antibody

IRX5 (IRX2A, IRXB2, Iroquois-class Homeodomain Protein IRX-5, Homeodomain Protein IRX-2A, Homeodomain Protein IRXB2, Iroquois Homeobox Protein 5) (FITC)

Cat. Num. AAA6147814

• Gene Names: IRX5; HMMS; IRXB2; IRX-2a
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IRX5; Monoclonal Antibody; IRX5 (IRX2A; IRXB2; Iroquois-class Homeodomain Protein IRX-5; Homeodomain Protein IRX-2A; Homeodomain Protein IRXB2; Iroquois Homeobox Protein 5) (FITC); anti-IRX5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 6B5
• Specificity: Recognizes human IRX5.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
• Sequence Length: 2398

• Applicable Applications for anti-IRX5 antibody: ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa204-264 from IRX5 (NP_005844) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: PQKPEDKADPEGPEAGGAEQKAASGCERLQGPPTPAGKETEGSLSDSDFKEPPSEGRLDAL
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (32.82kD).)

Western Blot (WB)

(IRX5 monoclonal antibody Western Blot analysis of IRX5 expression in HepG2)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to IRX5 on HeLa cell. [antibody concentration 10ug/ml])

Testing Data

(Detection limit for recombinant GST tagged IRX5 is ~0.03ng/ml as a capture antibody.)

Product Categories/Family for anti-IRX5 antibody

Antibodies; Transcription Factors; Homeobox (HOX)

NCBI and Uniprot Product Information

• NCBI GI #: 1653960833
• NCBI GeneID: 10265
• NCBI Accession #: NM_005853.6
• NCBI GenBank Nucleotide #: NM_005853
• UniProt Accession #: P78411
• NCBI Official Full Name: Homo sapiens iroquois homeobox 5 (IRX5), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: iroquois homeobox 5
• NCBI Official Symbol: IRX5
• NCBI Official Synonym Symbols: HMMS; IRXB2; IRX-2a
• NCBI Protein Information: iroquois-class homeodomain protein IRX-5
• UniProt Protein Name: Iroquois-class homeodomain protein IRX-5
• Protein Family: Iroquois-class homeodomain protein
• UniProt Gene Name: IRX5
• UniProt Synonym Gene Names: IRX2A; IRXB2
• UniProt Entry Name: IRX5_HUMAN

NCBI Description

This gene encodes a member of the iroquois homeobox gene family, which are involved in several embryonic developmental processes. Knockout mice lacking this gene show that it is required for retinal cone bipolar cell differentiation, and that it negatively regulates potassium channel gene expression in the heart to ensure coordinated cardiac repolarization. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]