Mouse Irx3 Monoclonal Antibody | anti-IRX3 antibody

Irx3 (Iroquois Homeobox Protein 3, Homeodomain Protein IrxB1, Iroquois Family Homeodomain Transcription Factor)

Cat. Num. AAA601475

• Gene Names: IRX3; IRX-1; IRXB1; FLJ99187
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Irx3; Monoclonal Antibody; Irx3 (Iroquois Homeobox Protein 3; Homeodomain Protein IrxB1; Iroquois Family Homeodomain Transcription Factor); Anti -Irx3 (Iroquois Homeobox Protein 3; anti-IRX3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C197
• Specificity: Recognizes human Irx3.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-IRX3 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blotting.; Dilution: Western Blotting: 1:1000-1:2000
• Immunogen: Partial sequence of recombinant full-length protein to human Irx3
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Product Categories/Family for anti-IRX3 antibody

Antibodies; Abs to Transcription Factors; Homeobox (HOX)

NCBI and Uniprot Product Information

• NCBI GI #: 226371735
• NCBI GeneID: 79191
• NCBI Accession #: NP_077312.2
• NCBI GenBank Nucleotide #: NM_024336.2
• UniProt Accession #: P78415; Q7Z4A4; Q7Z4A5; Q8IVC6; Q8N4G7
• Molecular Weight: 52,119 Da
• NCBI Official Full Name: iroquois-class homeodomain protein IRX-3
• NCBI Official Synonym Full Names: iroquois homeobox 3
• NCBI Official Symbol: IRX3
• NCBI Official Synonym Symbols: IRX-1; IRXB1; FLJ99187
• NCBI Protein Information: iroquois-class homeodomain protein IRX-3; OTTHUMP00000164257; homeodomain protein IRXB1; iroquois homeobox protein 3; iroquois-class homeodomain protein IRX-1
• UniProt Protein Name: Iroquois-class homeodomain protein IRX-3
• Protein Family: Iroquois-class homeodomain protein
• UniProt Gene Name: IRX3
• UniProt Synonym Gene Names: IRXB1
• UniProt Entry Name: IRX3_HUMAN

NCBI Description

IRX3 is a member of the Iroquois homeobox gene family (see IRX1; MIM 606197) and plays a role in an early step of neural development (Bellefroid et al., 1998 [PubMed 9427753]). Members of this family appear to play multiple roles during pattern formation of vertebrate embryos (Lewis et al., 1999 [PubMed 10370142]).[supplied by OMIM]

Uniprot Description

IRX3: Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells. Belongs to the TALE/IRO homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: axon; cytoplasm; nucleus

Molecular Function: sequence-specific DNA binding

Biological Process: negative regulation of neuron differentiation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; mesoderm development; positive regulation of neuron differentiation; metanephros development