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product-image-AAA179745_IHC10.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human small intestine using Nesfatin-1/Nucleobindin-2 Polyclonal Antibody at dilution of 1:950.)

Rabbit Nesfatin-1/Nucleobindin-2 Polyclonal Antibody | anti-NUCB2 antibody

Nesfatin-1/Nucleobindin-2 Polyclonal Antibody

Gene Names
NUCB2; NEFA; HEL-S-109
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
Nesfatin-1/Nucleobindin-2, Antibody; Nesfatin-1/Nucleobindin-2 Polyclonal Antibody; DNA-binding protein NEFA; NEFA; NEFAGastric cancer antigen Zg4; Nesfatin1; Nesfatin-1; NUCB2; nucleobindin 2; Nucleobindin-2; nucleobinding 2; anti-NUCB2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-NUCB2 antibody
IHC (Immunohistochemistry)
Immunogen
Recombinant Human Nesfatin-1/Nucleobindin-2 protein expressed by E coli
Preparation and Storage
Store at -20 degree C Valid for 12 months. Avoid freeze/thaw cycles. Protected from prolonged exposure to light.

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Human small intestine using Nesfatin-1/Nucleobindin-2 Polyclonal Antibody at dilution of 1:950.)

product-image-AAA179745_IHC10.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human small intestine using Nesfatin-1/Nucleobindin-2 Polyclonal Antibody at dilution of 1:950.)

IHC (Immunohistochemisry)

(Immunohistochemistry of paraffin-embedded Human gastric cancer using Nesfatin-1/Nucleobindin-2 Polyclonal Antibody at dilution of 1:950.)

product-image-AAA179745_IHC11.jpg IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human gastric cancer using Nesfatin-1/Nucleobindin-2 Polyclonal Antibody at dilution of 1:950.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded Rat brain using Nesfatin-1/Nucleobindin-2 Polyclonal Antibody at dilution of 1:950.)

product-image-AAA179745_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Rat brain using Nesfatin-1/Nucleobindin-2 Polyclonal Antibody at dilution of 1:950.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Mouse brain using Nesfatin-1/Nucleobindin-2 Polyclonal Antibody at dilution of 1:950.)

product-image-AAA179745_IHC15.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse brain using Nesfatin-1/Nucleobindin-2 Polyclonal Antibody at dilution of 1:950.)
Related Product Information for anti-NUCB2 antibody
Nesfatin-1 (NEFA Encoded Satiety and Fat-influencing protein 1) is a secreted, 10 kDa peptide derived from the translation product of the NUCB2 gene. Nesfatin-1 is associated with neurons involved in feeding (ARH and PVH) and fluid intake (SON and PVH), in beta cells of the pancreas, and in adipocytes. Its presence peripherally has an anorexigenic effect. Mature human Nesfatin-1 is 82 amino acids (aa) in length. It is the secreted N-terminus of a larger, intracellular 420 aa precursor termed NEFA/Nucleobindin-2/NUCB2. NUCB2 contains a signal sequence (aa 1- 24), Nesfatin-1 (aa 25-106), a DNA-binding site (aa 171-223), and two EF-hand regions (aa 241-276 and 293-328). Intracellular uncleaved NUCB2 is 45-50 kDa in size. There is a potential for multiple cleavages, generating Nesfatin-2 (aa 109-187) and Nesfatin-3 (aa 190-420). Human Nesfatin-1 shares 85% aa identity with mouse Nesfatin-1.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,196 Da
NCBI Official Full Name
nucleobindin-2
NCBI Official Synonym Full Names
nucleobindin 2
NCBI Official Symbol
NUCB2
NCBI Official Synonym Symbols
NEFA; HEL-S-109
NCBI Protein Information
nucleobindin-2; nesfatin-1; prepronefastin; prepronesfatin; nucleobinding 2; DNA-binding protein NEFA; gastric cancer antigen Zg4; epididymis secretory protein Li 109
UniProt Protein Name
Nucleobindin-2
UniProt Gene Name
NUCB2
UniProt Synonym Gene Names
NEFA
UniProt Entry Name
NUCB2_HUMAN

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Product Notes

The NUCB2 nucb2 (Catalog #AAA179745) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nesfatin-1/Nucleobindin-2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Nesfatin-1/Nucleobindin-2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the NUCB2 nucb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nesfatin-1/Nucleobindin-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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