Testing Data
(Detection limit for recombinant GST tagged IQGAP3 is ~0.1ng/ml as a capture antibody.)
Mouse anti-Human IQGAP3 Monoclonal Antibody | anti-IQGAP3 antibody
IQGAP3 (Ras GTPase-activating-like Protein IQGAP3, MGC10170, MGC10831, MGC1947) (MaxLight 550)
Reactivity
Human
Applications
Immunofluorescence, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
IQGAP3; Monoclonal Antibody; IQGAP3 (Ras GTPase-activating-like Protein IQGAP3; MGC10170; MGC10831; MGC1947) (MaxLight 550); anti-IQGAP3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
6E3
Specificity
Recognizes human IQGAP3.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Applicable Applications for anti-IQGAP3 antibody
FLISA, Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa602-699 OF human IQGAP3 with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
DTNTAQRMALGVAAINQAIKEGKAAQTERVLRNPAVALRGVVPDCANGYQRALESAMAKKQRPADTAFWVQHDMKDGTAYYFHLQTFQGIWEQPPGC
Conjugate
MaxLight550
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Testing Data
(Detection limit for recombinant GST tagged IQGAP3 is ~0.1ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged IQGAP3 is ~0.1ng/ml as a capture antibody.)
Western Blot (WB)
(Western Blot analysis of IQGAP3 expression in transfected 293T cell line by. Lane 1: IQGAP3 transfected lysate (184.6kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of IQGAP3 expression in transfected 293T cell line by. Lane 1: IQGAP3 transfected lysate (184.6kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-IQGAP3 antibody
MaxLight550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor546, 555, DyLight549, Cy3, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Product Categories/Family for anti-IQGAP3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
184,699 Da
NCBI Official Full Name
ras GTPase-activating-like protein IQGAP3
NCBI Official Synonym Full Names
IQ motif containing GTPase activating protein 3
NCBI Official Symbol
IQGAP3
NCBI Protein Information
ras GTPase-activating-like protein IQGAP3
UniProt Protein Name
Ras GTPase-activating-like protein IQGAP3
Protein Family
UniProt Gene Name
IQGAP3
UniProt Entry Name
IQGA3_HUMAN
Uniprot Description
IQGAP3:
Protein type: Motility/polarity/chemotaxis; GAPs; GAPs, Ras
Chromosomal Location of Human Ortholog: 1q21.3
Cellular Component: cytoplasm; intercellular junction; lateral plasma membrane
Molecular Function: calmodulin binding; Rho GTPase binding
Biological Process: activation of MAPK activity; Ras protein signal transduction; positive regulation of mammary gland epithelial cell proliferation; regulation of cell size; G1/S transition of mitotic cell cycle
Similar Products
Product Notes
The IQGAP3 iqgap3 (Catalog #AAA6212107) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IQGAP3 (Ras GTPase-activating-like Protein IQGAP3, MGC10170, MGC10831, MGC1947) (MaxLight 550) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IQGAP3 can be used in a range of immunoassay formats including, but not limited to, FLISA, Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the IQGAP3 iqgap3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IQGAP3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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