Mouse IQGAP1 Monoclonal Antibody | anti-IQGAP1 antibody

IQGAP1 (KIAA0051)

Cat. Num. AAA603267

• Gene Names: IQGAP1; SAR1; p195; KIAA0051; HUMORFA01
• Reactivity: Human, Monkey
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: IQGAP1; Monoclonal Antibody; IQGAP1 (KIAA0051); Anti -IQGAP1 (KIAA0051); anti-IQGAP1 antibody

• Host: Mouse
• Reactivity: Human, Monkey
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 3G170
• Specificity: This monoclonal antibody is specific for the Cdc42-binding protein IQGAP1 and does not exhibit any crossreactivity with the related IQGAP2 protein.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-IQGAP1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunoprecipitation.; Dilution: ELISA: 0.1-1ug/ml; Western Blot: 1-2ug/ml; Immunoprecipitation (native): 5ug/IP reaction
• Immunogen: A recombinant protein derived from the C-terminal fragment of human IQGAP1 protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-IQGAP1 antibody

Proteins of the IQGAP family have been identified as candidate effectors for the Rho family of GTPases. The domain structure of IQGAP family members suggests that these proteins may function as regulators of the cytoskeleton. In particular their sequences include 1) an actin-binding domain homologous to that found in calponin, 2) IQ motifs for interaction with calmodulin, and 3) a GTPase-binding domain. Two IQGAP isoforms have been identified, IQGAP1 and IQGAP2. These proteins share 62% sequence identity. The 189-kD IQGAP1 protein exhibits a broad tissue distribution and is especially enriched in placenta lung and kidney. On the other hand, the 180-kD IQGAP2 protein is restricted in its tissue distribution and appears to be expressed exclusively in liver. Both IQGAP1 and IQGAP2 bind to GTP-bound Cdc42 and inhibit the rate of GTP hydrolysis.

Product Categories/Family for anti-IQGAP1 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 116283871
• NCBI GeneID: 8826
• UniProt Accession #: Q6P1N4; P46940; Q05DN7; Q5FWG8; Q96PA3
• Molecular Weight: 107,539 Da
• NCBI Official Full Name: IQGAP1 protein
• NCBI Official Synonym Full Names: IQ motif containing GTPase activating protein 1
• NCBI Official Symbol: IQGAP1
• NCBI Official Synonym Symbols: SAR1; p195; KIAA0051; HUMORFA01
• NCBI Protein Information: ras GTPase-activating-like protein IQGAP1; OTTHUMP00000194748; RasGAP-like with IQ motifs
• UniProt Protein Name: IQGAP1 protein
• Protein Family: Ras GTPase-activating-like protein
• UniProt Gene Name: IQGAP1
• UniProt Entry Name: Q6P1N4_HUMAN

NCBI Description

This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq]

Uniprot Description

IQGAP1: Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth. Interacts with CDC42; the interaction is demonstrated with IQGAP1 in GTP-bound and in nucleotide-free state. Interacts with RAC1. Does not interact with RHOA. Interacts with TSG101. Interacts with PAK6. Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.

Protein type: GAPs, Ras; Motility/polarity/chemotaxis; GAPs

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: nucleoplasm; microtubule cytoskeleton; microtubule; neuron projection; extrinsic to internal side of plasma membrane; focal adhesion; growth cone; axon; cytoplasm; leading edge; plasma membrane; actin filament; midbody; cell junction; lateral plasma membrane; actin cytoskeleton

Molecular Function: calmodulin binding; protein serine/threonine kinase activator activity; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; protein complex binding; Rac GTPase binding; calcium ion binding; protein kinase binding; protein phosphatase binding; GTPase activator activity; GTPase inhibitor activity

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; positive regulation of protein kinase activity; negative regulation of dephosphorylation; small GTPase mediated signal transduction; negative regulation of catalytic activity; energy reserve metabolic process; platelet-derived growth factor receptor signaling pathway; signal transduction; regulation of insulin secretion; regulation of cytokine production; positive regulation of GTPase activity