Mouse anti-Human Interleukin 31 Receptor A Monoclonal Antibody | anti-IL-31RA antibody

Interleukin 31 Receptor A (Interleukin-31 Receptor Subunit alpha, IL-31 Receptor Subunit alpha, IL-31R Subunit alpha, IL-31R-alpha, IL-31RA, IL31RA, Cytokine Receptor-like 3, CRL3, CRL, gp130-Like Monocyte Receptor, Gp130-like Receptor, GLM-R, hGLM-R, GLM

Cat. Num. AAA6147795

• Gene Names: IL31RA; CRL; GPL; CRL3; GLMR; GLM-R; PLCA2; hGLM-R; IL-31RA; PRO21384
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Interleukin 31 Receptor A; Monoclonal Antibody; Interleukin 31 Receptor A (Interleukin-31 Receptor Subunit alpha; IL-31 Receptor Subunit alpha; IL-31R Subunit alpha; IL-31R-alpha; IL-31RA; IL31RA; Cytokine Receptor-like 3; CRL3; CRL; gp130-Like Monocyte Receptor; Gp130-like Receptor; GLM-R; hGLM-R; GLM; anti-IL-31RA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3A10
• Specificity: Recognizes human IL31RA.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
• Sequence Length: 764

• Applicable Applications for anti-IL-31RA antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant protein corresponding to aa21-121 from human IL31RA (NP_620586) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: LPAKPENISCVYYYRKNLTCTWSPGKETSYTQYTVKRTYAFGEKHDNCTTNSSTSENRASCSFFLPRITIPDNYTIEVEAENGDGVIKSHMTYWRLENIA
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (37.11kD).)

Western Blot (WB)

(Western Blot analysis of IL31RA expression in transfected 293T cell line by IL31RA monoclonal antibody. Lane 1: IL31RA transfected lysate (83kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to IL31RA on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3ug/ml].)

Western Blot (WB)

(Western blot analysis of IL31RA over-expressed 293 cell line, cotransfected with IL31RA Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with IL31RA monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Product Categories/Family for anti-IL-31RA antibody

Antibodies; Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 115430248
• NCBI GeneID: 133396
• NCBI Accession #: NP_620586
• NCBI GenBank Nucleotide #: NM_139017
• UniProt Accession #: Q8NI17
• NCBI Official Full Name: interleukin-31 receptor subunit alpha isoform 1
• NCBI Official Synonym Full Names: interleukin 31 receptor A
• NCBI Official Symbol: IL31RA
• NCBI Official Synonym Symbols: CRL; GPL; CRL3; GLMR; GLM-R; PLCA2; hGLM-R; IL-31RA; PRO21384
• NCBI Protein Information: interleukin-31 receptor subunit alpha
• UniProt Protein Name: Interleukin-31 receptor subunit alpha
• UniProt Gene Name: IL31RA
• UniProt Synonym Gene Names: CRL3; GPL; IL-31 receptor subunit alpha; IL-31R subunit alpha; IL-31R-alpha; IL-31RA; hGLM-R; Gp130-like receptor

NCBI Description

The protein encoded by this gene belongs to the type I cytokine receptor family. This receptor, with homology to gp130, is expressed on monocytes, and is involved in IL-31 signaling via activation of STAT-3 and STAT-5. It functions either as a monomer, or as part of a receptor complex with oncostatin M receptor (OSMR). Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

IL31RA: Associates with OSMR to form the interleukin-31 receptor which activates STAT3 and to a lower extent STAT1 and STAT5. May function in skin immunity. Defects in IL31RA are the cause of amyloidosis primary localized cutaneous type 2 (PLCA2). PLCA2 is primary amyloidosis characterized by localized cutaneous amyloid deposition. This condition usually presents with itching (especially on the lower legs) and visible changes of skin hyperpigmentation and thickening that may be exacerbated by chronic scratching and rubbing. Primary localized cutaneous amyloidosis is often divided into macular and lichen subtypes although many affected individuals often show both variants coexisting. Lichen amyloidosis characteristically presents as a pruritic eruption of grouped hyperkeratotic papules with a predilection for the shins, calves, ankles and dorsa of feet and thighs. Papules may coalesce to form hyperkeratotic plaques that can resemble lichen planus, lichen simplex or nodular prurigo. Macular amyloidosis is characterized by small pigmented macules that may merge to produce macular hyperpigmentation, sometimes with a reticulate or rippled pattern. In macular and lichen amyloidosis, amyloid is deposited in the papillary dermis in association with grouped colloid bodies, thought to represent degenerate basal keratinocytes. The amyloid deposits probably reflect a combination of degenerate keratin filaments, serum amyloid P component, and deposition of immunoglobulins. Belongs to the type I cytokine receptor family. Type 2 subfamily. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: axon; cell junction; external side of plasma membrane; integral component of membrane; intracellular; plasma membrane; presynaptic membrane

Molecular Function: cytokine binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein kinase binding; transcription coactivator activity

Biological Process: acute inflammatory response to antigenic stimulus; cytokine-mediated signaling pathway; defense response; glandular epithelial cell differentiation; homeostatic process; JAK-STAT cascade; macrophage differentiation; MAPK cascade; monocyte differentiation; negative regulation of apoptosis; negative regulation of macrophage activation; positive regulation of cell proliferation; positive regulation of transcription, DNA-templated; positive regulation of tyrosine phosphorylation of STAT protein; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Amyloidosis, Primary Localized Cutaneous, 2