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Western Blot (WB) (Western Blot; Sample: Recombinant IL28A, Human.)

Mouse anti-Human Interleukin 28A (IL28A) Monoclonal Antibody | anti-IL28A antibody

Monoclonal Antibody to Interleukin 28A (IL28A)

Gene Names
IFNL2; IL28A; IL-28A
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Protein A + Protein G affinity chromatography
Synonyms
Interleukin 28A (IL28A); Monoclonal Antibody; Monoclonal Antibody to Interleukin 28A (IL28A); IFNL2; Interferon; Lambda 2; Cytokine Zcyto20; anti-IL28A antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C15
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-IL28A antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Organism Species
Homo sapiens (Human)
Source
Monoclonal antibody preparation
Traits
Liquid
Cross Reactivity
Human
Immunogen
Recombinant IL28A (Val26~Val200 (Accession # Q8IZJ0)) expressed in E.coli (MBS2030729)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 24 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot; Sample: Recombinant IL28A, Human.)

Western Blot (WB) (Western Blot; Sample: Recombinant IL28A, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Lung cancer Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Lung cancer Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Human Stomach cancer Tissue; Primary Ab: 20ug/ml Mouse Anti-Human IL28A AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035))

Immunohistochemistry (IHC) (DAB staining on IHC-P;Samples: Human Stomach cancer Tissue; Primary Ab: 20ug/ml Mouse Anti-Human IL28A AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
interferon lambda-2
NCBI Official Synonym Full Names
interferon lambda 2
NCBI Official Symbol
IFNL2
NCBI Official Synonym Symbols
IL28A; IL-28A
NCBI Protein Information
interferon lambda-2
UniProt Protein Name
Interferon lambda-2
UniProt Gene Name
IFNL2
UniProt Synonym Gene Names
IL28A; ZCYTO20; IFN-lambda-2; IL-28A

NCBI Description

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B (IL28B), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]

Uniprot Description

IL28A: Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the IL-28/IL-29 family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; intracellular

Molecular Function: cytokine activity; receptor binding

Biological Process: defense response to virus; innate immune response; JAK-STAT cascade; mucosal immune response; positive regulation of immune response; regulation of T cell activation

Research Articles on IL28A

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Product Notes

The IL28A ifnl2 (Catalog #AAA2085905) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Interleukin 28A (IL28A) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 28A (IL28A) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the IL28A ifnl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 28A (IL28A), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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