Western Blot (WB)
(Western Blot; Sample: Mouse Placenta lysate; Primary Ab: 5ug/ml Mouse Anti-Mouse IL10Ra AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Mouse anti-Mouse Interleukin 10 Receptor Alpha (IL10Ra) Monoclonal Antibody | anti-IL10Ra antibody
Monoclonal Antibody to Interleukin 10 Receptor Alpha (IL10Ra)
Gene Names
Il10ra; Il10r; CDw210; CDw210a; mIL-10R; AW553859
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Protein A + Protein G Affinity Chromatography
Synonyms
Interleukin 10 Receptor Alpha (IL10Ra); Monoclonal Antibody; Monoclonal Antibody to Interleukin 10 Receptor Alpha (IL10Ra); CDW210A; CD210A; IL10R-A; IL10-RA; CD210-A; HIL-10R; IL-10R1; IL10R; Cluster Of Differentiation W210A; anti-IL10Ra antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C7
Specificity
The antibody is a mouse monoclonal antibody raised against IL10Ra. It has been selected for its ability to recognize IL10Ra in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G Affinity Chromatography
Form/Format
Liquid; 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Leu17~Ile241
Applicable Applications for anti-IL10Ra antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.2-2ug/mL; 1:500-5000
IHC: 5-20ug/mL; 1:50-200
IHC: 5-20ug/mL; 1:50-200
Immunogen Sequence
Leu17~Ile241
Cross Reactivity
Mouse
Conjugation
None
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot; Sample: Mouse Placenta lysate; Primary Ab: 5ug/ml Mouse Anti-Mouse IL10Ra AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot; Sample: Mouse Placenta lysate; Primary Ab: 5ug/ml Mouse Anti-Mouse IL10Ra AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Cerebrum Tissue; Primary Ab: 10ug/ml Mouse Anti-Mouse IL10Ra Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Cerebrum Tissue; Primary Ab: 10ug/ml Mouse Anti-Mouse IL10Ra Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Product Categories/Family for anti-IL10Ra antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
64,248 Da
NCBI Official Full Name
interleukin-10 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 10 receptor, alpha
NCBI Official Symbol
Il10ra
NCBI Official Synonym Symbols
Il10r; CDw210; CDw210a; mIL-10R; AW553859
NCBI Protein Information
interleukin-10 receptor subunit alpha; IL-10 receptor subunit alpha; IL-10R subunit 1; IL-10R subunit alpha; IL-10R1; IL-10RA; interleukin-10 receptor subunit 1
UniProt Protein Name
Interleukin-10 receptor subunit alpha
Protein Family
UniProt Gene Name
Il10ra
UniProt Synonym Gene Names
Il10r; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; IL-10R subunit 1; IL-10R1
UniProt Entry Name
I10R1_MOUSE
Uniprot Description
IL10RA: a receptor for interleukin 10 structurally related to interferon receptors. Mediates the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases.
Protein type: Receptor, cytokine; Membrane protein, integral
Cellular Component: membrane; integral to membrane; plasma membrane
Molecular Function: interleukin-10 binding; interleukin-10 receptor activity
Biological Process: cytokine and chemokine mediated signaling pathway
Research Articles on IL10Ra
Similar Products
Product Notes
The IL10Ra il10ra (Catalog #AAA2111625) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Interleukin 10 Receptor Alpha (IL10Ra) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 10 Receptor Alpha (IL10Ra) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.2-2ug/mL; 1:500-5000 IHC: 5-20ug/mL; 1:50-200. Researchers should empirically determine the suitability of the IL10Ra il10ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Leu17~Ile2 41. It is sometimes possible for the material contained within the vial of "Interleukin 10 Receptor Alpha (IL10Ra), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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