Mouse Integrin beta1, Activating Monoclonal Antibody | anti-ITGB1 antibody

Integrin beta1, Activating

Cat. Num. AAA603671

• Reactivity: Human, Monkey, Rabbit
• Applications: ELISA, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Integrin beta1; Activating; Monoclonal Antibody; Anti -Integrin beta1; anti-ITGB1 antibody

• Host: Mouse
• Reactivity: Human, Monkey, Rabbit
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2Q849
• Specificity: Recognizes human Integrin beta 1. Reacts with calcium-dependent epitopes on the integrin b1 subunit. When bound to b1, activates b1 dependent binding to extra cellular matrix substrates.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.1. No preservatives.

• Applicable Applications for anti-ITGB1 antibody: ELISA (EL/EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, FACS and ELISA.; Dilution: Immunocytochemistry: 2ug/ml (use on numerous cell lines as long as Ca2+ is present),; Immunoprecipitation: 5ug/400ul lysate (0.5% triton PBS plus Ca++); FACS: 2-5ug/10e6 cells, in the presence of Ca++.; Stimulation of cells: 2-10ug/ml per 10e6 cells. Binding buffer must contain 1mM Ca++; Immunohistochemistry: For use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and paraffin embedded (1:500, citrate HEIR treated, Qin et al 2003). Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.; Optimal dilution determined by the researcher.
• Immunogen: Human IL-1b activated endothelial cells.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-ITGB1 antibody

Antibodies; Abs to Integrin

NCBI and Uniprot Product Information

• NCBI GI #: 298231107
• NCBI GeneID: 374058
• NCBI Accession #: NP_001034343.2
• NCBI GenBank Nucleotide #: NM_001039254.2
• UniProt Accession #: P07228; Q8AV89
• Molecular Weight: 88,554 Da
• NCBI Official Full Name: integrin beta-1
• NCBI Official Symbol: ITGB1
• NCBI Protein Information: integrin beta-1; CSAT antigen; JG22 antigen; RGD-receptor
• UniProt Protein Name: Integrin beta-1
• Protein Family: Integrin
• UniProt Gene Name: ITGB1
• UniProt Entry Name: ITB1_CHICK

Uniprot Description

ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. . Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Receptor, misc.; Membrane protein, integral; Cell surface

Cellular Component: lamellipodium; plasma membrane; melanosome; integrin complex; cell junction

Molecular Function: protein binding; metal ion binding; receptor activity

Biological Process: integrin-mediated signaling pathway; multicellular organismal development; cell-matrix adhesion