Mouse anti-Human Integrin alphaV, beta5 Monoclonal Antibody | anti-ITGAV antibody

Integrin alphaV, beta5 (MaxLight 650)

Cat. Num. AAA6246510

• Gene Names: ITGAV; CD51; MSK8; VNRA; VTNR
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunocytochemistry, Immunoprecipitation
• Purity: Purified
• Synonyms: Integrin alphaV; beta5; Monoclonal Antibody; beta5 (MaxLight 650); anti-ITGAV antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 2Q873
• Specificity: Recognizes human integrin alphaVbeta5. Does not recognize integrin alphaVbeta3 or alpha5beta1. Binds to the alphaVbeta5 complex. Has a Kd of 2.8nM in Mn2+, 4.0nM in Ca2+and 2.9nM in buffer lacking cation. Thus, cations do not significantly affect the binding to alphaVbeta5. Does not block ligand binding to purified alphaVbeta5, nor does it block cell adhesion mediated through this receptor.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-ITGAV antibody: Flow Cytometry (FC/FACS), FLISA, Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Immunogen: Human integrin alphaVbeta5 purified from placental tissue and depleted of integrin alphaVbeta3.
• Conjugate: MaxLight650
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Product Categories/Family for anti-ITGAV antibody

Antibodies; Integrin

References

1. Hybridoma (1995) 14: 545-550. 2. J. Cell. Physiol. (1996) 168: 521-531.

NCBI and Uniprot Product Information

• NCBI GI #: 223468595
• NCBI GeneID: 3685
• NCBI Accession #: NP_001138471.1
• NCBI GenBank Nucleotide #: NM_001144999.2
• UniProt Accession #: P06756; Q53SK4; Q59EB7; Q6LD15; A0AV67; B0LPF4; B7Z883; D3DPG8; E7EWZ6
• Molecular Weight: 116,038 Da
• NCBI Official Full Name: integrin alpha-V isoform 2
• NCBI Official Synonym Full Names: integrin, alpha V
• NCBI Official Symbol: ITGAV
• NCBI Official Synonym Symbols: CD51; MSK8; VNRA; VTNR
• NCBI Protein Information: integrin alpha-V; integrin alphaVbeta3; vitronectin receptor subunit alpha; antigen identified by monoclonal antibody L230; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• UniProt Protein Name: Integrin alpha-V
• Protein Family: Integrin
• UniProt Gene Name: ITGAV
• UniProt Synonym Gene Names: MSK8; VNRA
• UniProt Entry Name: ITAV_HUMAN

NCBI Description

This gene encodes a protein that is a member of the integrin superfamily. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein undergoes post-translational cleavage to yield disulfide-linked heavy and light chains that combine with multiple integrin beta chains to form different integrins. This protein has been shown to heterodimerize with beta 1, beta 3, beta 5, beta 6, and beta 8; the heterodimer of alpha v and beta 3 is the Vitronectin receptor. This protein interacts with several extracellular matrix proteins to mediate cell adhesion and may play a role in cell migration. It is proposed that this protein may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, May 2013]

Uniprot Description

ITGAV: The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Belongs to the integrin alpha chain family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q31-q32

Cellular Component: filopodium membrane; focal adhesion; cell surface; membrane; integral to plasma membrane; microvillus membrane; plasma membrane; phagocytic vesicle; integrin complex; external side of plasma membrane

Molecular Function: voltage-gated calcium channel activity; viral receptor activity; protein binding; opsonin binding; insulin-like growth factor I binding; protein kinase C binding; protease binding; extracellular matrix binding; transforming growth factor beta binding; metal ion binding; fibronectin binding

Biological Process: negative regulation of lipid transport; axon guidance; entry of virus into host cell; extracellular matrix organization and biogenesis; positive regulation of cell adhesion; cell-matrix adhesion; negative chemotaxis; entry of symbiont into host cell by promotion of host phagocytosis; regulation of phagocytosis; antigen processing and presentation of peptide antigen via MHC class I; positive regulation of cell proliferation; antigen processing and presentation of exogenous peptide antigen via MHC class I; angiogenesis; cell adhesion; cell growth; integrin-mediated signaling pathway; cell migration; negative regulation of lipoprotein metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; cell-substrate adhesion; heterotypic cell-cell adhesion; negative regulation of low-density lipoprotein receptor biosynthetic process; positive regulation of osteoblast proliferation; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration; apoptotic cell clearance; positive regulation of cell migration