Insulin C-terminal Pentapeptide of B-chain Monoclonal Antibody | anti-INS antibody

MAb to Insulin C-terminal

Cat. Num. AAA310709

• Gene Names: INS; ILPR; IRDN; IDDM2; MODY10
• Applications: ELISA
• Purity: >90% pure (SDS-PAGE). Protein A chromatography
• Synonyms: Insulin C-terminal Pentapeptide of B-chain; Monoclonal Antibody; MAb to Insulin C-terminal; anti-INS antibody

• Host: Mouse; Hybridization of X63-Ag8-653 myeloma cells with spleen cells from BALB/c mice.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Specificity: Insulin, human. Reacts with c-terminal pentapeptide of insulin beta-chain. Cross-reacts with human proinsulin, bovine insulin (30%) and porcine insulin.
• Purity/Purification: >90% pure (SDS-PAGE). Protein A chromatography
• Form/Format: Purified, Liquid; PBS, pH 7.4
• Concentration: 8.2 mg/ml (OD280nm, E^0.1% = 1.4) (varies by lot)
• Sequence Length: 110

• Applicable Applications for anti-INS antibody: EIA/ELISA
• Application Notes: Each laboratory should determined an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
• Immunogen: Purified human insulin
• Affinit Constant: 1 x 10⁹M^-1
• Preservative: 0.09% Sodium azide
• Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Preparation and Storage: Store at 2-8°C.

Related Product Information for anti-INS antibody

Mab to Insulin C-Terminal
Monoclonal Antibody to Human Insulin C-terminal

Product Categories/Family for anti-INS antibody

Monoclonal Antibodies to Hormones and Steroids

NCBI and Uniprot Product Information

• NCBI GI #: 386828
• NCBI GeneID: 3630
• UniProt Accession #: P01308; Q5EEX2
• NCBI Official Full Name: insulin
• NCBI Official Synonym Full Names: insulin
• NCBI Official Symbol: INS
• NCBI Official Synonym Symbols: ILPR; IRDN; IDDM2; MODY10
• NCBI Protein Information: insulin; proinsulin; OTTHUMP00000011161; OTTHUMP00000011162; OTTHUMP00000196036; OTTHUMP00000196038; OTTHUMP00000217519
• UniProt Protein Name: Insulin
• Protein Family: Insulin
• UniProt Gene Name: INS
• UniProt Entry Name: INS_HUMAN

NCBI Description

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Uniprot Description

Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family.

Protein type: Secreted; Hormone; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; endoplasmic reticulum lumen; Golgi lumen; extracellular region

Molecular Function: identical protein binding; insulin-like growth factor receptor binding; protein binding; protease binding; hormone activity; insulin receptor binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of vasodilation; glucose homeostasis; activation of NF-kappaB transcription factor; negative regulation of protein oligomerization; positive regulation of glucose import; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; activation of protein kinase B; positive regulation of nitric-oxide synthase activity; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; regulation of amino acid metabolic process; negative regulation of protein catabolic process; positive regulation of cell differentiation; negative regulation of glycogen catabolic process; wound healing; positive regulation of cellular protein metabolic process; positive regulation of glycogen biosynthetic process; glucose transport; regulation of protein secretion; negative regulation of lipid catabolic process; negative regulation of vasodilation; regulation of transcription, DNA-dependent; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; alpha-beta T cell activation; negative regulation of fatty acid metabolic process; endocrine pancreas development; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; insulin receptor signaling pathway; energy reserve metabolic process; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cell migration; positive regulation of cytokine secretion

Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10