anti-Human Insulin Monoclonal Antibody | anti-INS antibody

MAb to Insulin

Cat. Num. AAA312647

• Gene Names: INS; ILPR; IRDN; IDDM2; MODY10
• Reactivity: Human
• Applications: ELISA
• Purity: >90% pure (SDS-PAGE). Protein A chromatography
• Synonyms: Insulin; Monoclonal Antibody; MAb to Insulin; Monoclonal Antibody to Human Insulin; anti-INS antibody

• Host: Host: Mouse. Hybridization of X63-Ag8-653 myeloma cells with spleen cells from Balb/c mice.; Source: Ascites
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 7F5
• Specificity: Insulin; Human insulin. Cross reacts with human proinsulin, bovine insulin (30%) and porcine insulin. Does not cross react with free C-peptide.
• Purity/Purification: >90% pure (SDS-PAGE). Protein A chromatography
• Form/Format: Purified, Liquid
• Concentration: 7.2mg/ml (OD280nm, E^0.1% = 1.4) (varies by lot)
• Sequence Length: 110

• Applicable Applications for anti-INS antibody: EIA/ELISA
• Immunogen: Purified Human insulin
• Affinity Constant: Kass = 1.0 x 10^9 M^-1
• Buffer: PBS, pH 7.4
• Preservative: 0.1% Sodium azide
• Important Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
• Preparation and Storage: Store at 2 to 8 degree C.

Product Categories/Family for anti-INS antibody

Monoclonal Antibodies to Hormones and Steroids

NCBI and Uniprot Product Information

• NCBI GI #: 386828
• NCBI GeneID: 3630
• UniProt Accession #: P01308; Q5EEX2
• Molecular Weight: 11,981 Da
• NCBI Official Full Name: insulin
• NCBI Official Synonym Full Names: insulin
• NCBI Official Symbol: INS
• NCBI Official Synonym Symbols: ILPR; IRDN; IDDM2; MODY10
• NCBI Protein Information: insulin; proinsulin; OTTHUMP00000011161; OTTHUMP00000011162; OTTHUMP00000196036; OTTHUMP00000196038; OTTHUMP00000217519
• UniProt Protein Name: Insulin
• Protein Family: Insulin
• UniProt Gene Name: INS
• UniProt Entry Name: INS_HUMAN

NCBI Description

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Uniprot Description

Function: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Subunit structure: Heterodimer of a B chain and an A chain linked by two disulfide bonds.

Subcellular location: Secreted.

Involvement in disease: Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [

MIM:176730]. Ref.21 Ref.23 Ref.24 Ref.25Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [

MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Ref.34Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [

MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy. Ref.32 Ref.33Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [

MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Ref.33 Ref.34 Ref.35

Pharmaceutical use: Available under the names Humulin or Humalog (Eli Lilly) and Novolin (Novo Nordisk). Used in the treatment of diabetes. Humalog is an insulin analog with 52-Lys-Pro-53 instead of 52-Pro-Lys-53.

Sequence similarities: Belongs to the insulin family.

Sequence caution: The sequence AAA59179.1 differs from that shown. Reason: Erroneous gene model prediction.