Immunohistochemistry (IHC)
(IHC analysis of in formalin-fixed paraffin embedded human pancreas using (1:500), peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of cells.)
Mouse Insulin Monoclonal Antibody
Insulin (AP)
Reactivity
Bovine, Human, Mouse, Porcine
Applications
Immunohistochemistry
Purity
Purified by Protein G Affinity Chromatography
Synonyms
Insulin; Monoclonal Antibody; Insulin (AP); anti-Insulin antibody
Host
Mouse
Reactivity
Bovine, Human, Mouse, Porcine
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2D11-H5
Specificity
Recognizes porcine Insulin. Species Crossreactivity: human, bovine and mouse
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-Insulin antibody
Immunohistochemistry (IHC)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Purified porcine insulin, conjugatd to KLH. Cellular Localization: Cytoplasmic
Conjugate
AP
Cellular Localization
Cytoplasmic
Positive Control
Pancreas
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Immunohistochemistry (IHC)
(IHC analysis of in formalin-fixed paraffin embedded human pancreas using (1:500), peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of cells.)
Immunohistochemistry (IHC)
(IHC analysis of in formalin-fixed paraffin embedded human pancreas using (1:500), peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of cells.)
Related Product Information for anti-Insulin antibody
Recognizes a polypeptide which is identified as insulin, a 51aa polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.
Product Categories/Family for anti-Insulin antibody
References
1. de la Tour, D., et al. 2001. Mol. Endoc. 15: 476-483. 2. Rajagopal, J., et al. 2003. Science 299: 363. 3. Morisset, J., et al. 2003. J. Histochem. Cytochem. 51: 1501-1513.
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Product Notes
The Insulin (Catalog #AAA6122267) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Insulin (AP) reacts with Bovine, Human, Mouse, Porcine and may cross-react with other species as described in the data sheet. AAA Biotech's Insulin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Insulin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Insulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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