Mouse iNOS Monoclonal Antibody | anti-iNOS antibody

iNOS (C-terminal region) M575 Antibody

Cat. Num. AAA474476

• Gene Names: NOS2; NOS; INOS; NOS2A; HEP-NOS
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA, Immunoprecipitation, Immunocytochemistry
• Synonyms: iNOS; Monoclonal Antibody; iNOS (C-terminal region) M575 Antibody; iNOS (C-terminal region); NOSi m2; anti-iNOS antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: M575
• Specificity: The antibody detects a 130kDa* protein on SDS-PAGE immunoblots of mouse macrophages (RAW264.7) treated with IFNgamma and LPS, or J774A.1 cells treated with LPS only.
• Form/Format: Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-iNOS antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Application Notes: ELISA: 1:2000; ICC: 1:200; IP: 1:100; WB: 1:1000
• Immunogen: Clone (M575) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human iNOS. The human iNOS sequence used has high homology with similar regions in rat and mouse iNOS.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot analysis of mouse macrophage (RAW264.7) untreated (lane 1) or treated with IFNgamma (10 ng/ml) and LPS (1ug/ml) for 12 hrs (lanes 2). The blot was probed with mouse monoclonal anti-iNOS M575 at 1:1000 (lanes 1 & 2).)

Related Product Information for anti-iNOS antibody

Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (also designated bNOS, NOS-I), whose activity was first identified in neurons, and eNOS (also designated ecNOS, NOS-III) first identified in endothelial cells. The inducible form of NOS, iNOS (also designated NOS-II), is Ca2+ independent and is expressed in a broad range of cell types. This form of NOS is induced after stimulation with cytokines and exposure to microbial products.

References

Musicki, B. et al. (2005) Proc. Natl. Acad. Sci.102(33):11870.
Kleinert, H. et al. (2003) Biol Chem. 384(10-11):1343.
Xie, Q.W. et al. (1992) Science 256:225.

NCBI and Uniprot Product Information

• NCBI GI #: 24041029
• NCBI GeneID: 4843
• NCBI Accession #: NP_000616.3
• NCBI GenBank Nucleotide #: NM_000625.4
• UniProt Accession #: P35228; O60757; O94994; Q16263; Q16692; Q4TTS5; Q9UD42; A1L3U5; B7ZLY2
• Molecular Weight: 131,117 Da
• NCBI Official Full Name: nitric oxide synthase, inducible
• NCBI Official Synonym Full Names: nitric oxide synthase 2, inducible
• NCBI Official Symbol: NOS2
• NCBI Official Synonym Symbols: NOS; INOS; NOS2A; HEP-NOS
• NCBI Protein Information: nitric oxide synthase, inducible; NOS type II; NOS, type II; inducible NOS; hepatocyte NOS; inducible NO synthase; nitric oxide synthase, macrophage; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes)
• UniProt Protein Name: Nitric oxide synthase, inducible
• Protein Family: Inositol 3-kinase
• UniProt Gene Name: NOS2
• UniProt Synonym Gene Names: NOS2A; HEP-NOS; Inducible NOS; iNOS
• UniProt Entry Name: NOS2_HUMAN

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Uniprot Description

iNOS: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cortical cytoskeleton; perinuclear region of cytoplasm; cytoplasm; peroxisome; intracellular; nucleus; cytosol

Molecular Function: calmodulin binding; protein binding; protein homodimerization activity; FAD binding; NADPH-hemoprotein reductase activity; FMN binding; nitric-oxide synthase activity; iron ion binding; heme binding; NADP binding; receptor binding

Biological Process: circadian rhythm; interaction with host; superoxide metabolic process; positive regulation of guanylate cyclase activity; positive regulation of killing of cells of another organism; defense response to Gram-negative bacterium; positive regulation of vasodilation; regulation of cell proliferation; regulation of cellular respiration; positive regulation of leukocyte mediated cytotoxicity; innate immune response in mucosa; response to bacterium; defense response to bacterium; peptidyl-cysteine S-nitrosylation; response to hypoxia; arginine catabolic process; negative regulation of blood pressure; blood coagulation; negative regulation of protein catabolic process; inflammatory response; nitric oxide biosynthetic process; regulation of insulin secretion; nitric oxide mediated signal transduction

Disease: Hypertension, Essential; Malaria, Susceptibility To