Western Blot (WB)
(ING5 antibody (mAb) tested by immunoprecipitation. 5 ug of ING5 antibody (Lane 1) or an unrelated mouse IgG (Lane 2) was used to immunoprecipitate ING5 from 300 ug of HEK293 whole cell lysate. The immunoprecipitated protein was detected by Western blotting using a the same ING5 antibody at 2 ug/ml (crossreaction between heavy and light chains of the antibody is observed and is common).)
Rat anti-Human ING5 Monoclonal Antibody | anti-ING5 antibody
ING5 antibody (mAb)(Clone 7A11)
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein G Chromatography
Synonyms
ING5; Monoclonal Antibody; ING5 antibody (mAb)(Clone 7A11); Inhibitor of growth protein 5; p28ING5; anti-ING5 antibody
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
7A11
Purity/Purification
Protein G Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-ING5 antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
WB: 0.5-2ug/ml
IP: 5ug/ml
This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody
WB: 0.5-2ug/ml
IP: 5ug/ml
This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody
Immunogen
This antibody was raised against full length recombinant human ING5 (Inhibitor of growth protein 5) protein.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(ING5 antibody (mAb) tested by immunoprecipitation. 5 ug of ING5 antibody (Lane 1) or an unrelated mouse IgG (Lane 2) was used to immunoprecipitate ING5 from 300 ug of HEK293 whole cell lysate. The immunoprecipitated protein was detected by Western blotting using a the same ING5 antibody at 2 ug/ml (crossreaction between heavy and light chains of the antibody is observed and is common).)
Western Blot (WB)
(ING5 antibody (mAb) tested by immunoprecipitation. 5 ug of ING5 antibody (Lane 1) or an unrelated mouse IgG (Lane 2) was used to immunoprecipitate ING5 from 300 ug of HEK293 whole cell lysate. The immunoprecipitated protein was detected by Western blotting using a the same ING5 antibody at 2 ug/ml (crossreaction between heavy and light chains of the antibody is observed and is common).)
Western Blot (WB)
(ING5 antibody (mAb) tested by Western blot 20 ug of HEK293 whole cell extract was run on SDS-PAGE and probed with antibody at 0.5 ug/ml.)
Western Blot (WB)
(ING5 antibody (mAb) tested by Western blot 20 ug of HEK293 whole cell extract was run on SDS-PAGE and probed with antibody at 0.5 ug/ml.)
Related Product Information for anti-ING5 antibody
Short Description: Our ING5 antibody (mAb)(Clone 7A11) was raised in a Rat host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: ING5 (Inhibitor of Growth 5) possesses different structural and functional elements, including a PHD (plant homeodomain), an NCR (novel conserved region), a LZL (leucine zipper like) finger, and two NLS (nuclear localization sequence) motifs. ING proteins bind to methylated core histones through their PHD motif, notably ING5 reads histone H3 when di- or trimethylated at lysine 4 (H3K4me2/3). ING5 is a subunit of two distinct histone acetyltransferase (HAT) complexes with HBO1 or MOZ/MORF as the two catalytic subunits. These complexes contain two different alternative scaffolding proteins, i.e. a BRPF protein or a JADE protein, that determine their specificity towards different core histones. ING complexes appear to participate in transcription, for example by interacting with transcriptional regulators such as the tumor suppressor p53 and through the ability of ING5 to recognize H3K4me3, a mark associated with open chromatin and transcribed genes.
Background: ING5 (Inhibitor of Growth 5) possesses different structural and functional elements, including a PHD (plant homeodomain), an NCR (novel conserved region), a LZL (leucine zipper like) finger, and two NLS (nuclear localization sequence) motifs. ING proteins bind to methylated core histones through their PHD motif, notably ING5 reads histone H3 when di- or trimethylated at lysine 4 (H3K4me2/3). ING5 is a subunit of two distinct histone acetyltransferase (HAT) complexes with HBO1 or MOZ/MORF as the two catalytic subunits. These complexes contain two different alternative scaffolding proteins, i.e. a BRPF protein or a JADE protein, that determine their specificity towards different core histones. ING complexes appear to participate in transcription, for example by interacting with transcriptional regulators such as the tumor suppressor p53 and through the ability of ING5 to recognize H3K4me3, a mark associated with open chromatin and transcribed genes.
Product Categories/Family for anti-ING5 antibody
NCBI and Uniprot Product Information
Molecular Weight
30kDa
Protein Family
Similar Products
Product Notes
The ING5 (Catalog #AAA389056) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The ING5 antibody (mAb)(Clone 7A11) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ING5 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Applications Validated: WB: 0.5-2ug/ml IP: 5ug/ml This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody. Researchers should empirically determine the suitability of the ING5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ING5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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