Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human IL7Ra(CD127) expression on human whole blood lymphocytes. Cells were stained with APC-conjugated anti-Human IL7Ra(CD127) and FITC-conjugated anti-Human CD3. The dot plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Rabbit anti-Human IL7R alpha/CD127 Monoclonal Antibody | anti-IL7R antibody
Recombinant Anti-IL7R alpha/CD127 Antibody (APC), Rabbit Monoclonal
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
IL7R alpha/CD127; Monoclonal Antibody; Recombinant Anti-IL7R alpha/CD127 Antibody (APC); Rabbit Monoclonal; IL7R/IL-7R/CD127 Antibody (APC); Rabbit MAb; interleukin 7 receptor; Anti-CD127 Antibody; Anti-CDW127 Antibody; Anti-IL-7R Antibody; Anti-IL-7R-alpha Antibody; Anti-IL7RA Antibody; Anti-ILRA Antibody; anti-IL7R antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
014
Specificity
Human IL7R/IL-7R/CD127
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
5ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-IL7R antibody
Flow Cytometry (FC/FACS/FCM)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human IL7R/IL-7R/CD127 Protein
Conjugation
APC
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human IL7R/IL-7R/CD127 (rh IL7R/IL-7R/CD127) and conjugated with APC under optimum conditions, the unreacted APC was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human IL7Ra(CD127) expression on human whole blood lymphocytes. Cells were stained with APC-conjugated anti-Human IL7Ra(CD127) and FITC-conjugated anti-Human CD3. The dot plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human IL7Ra(CD127) expression on human whole blood lymphocytes. Cells were stained with APC-conjugated anti-Human IL7Ra(CD127) and FITC-conjugated anti-Human CD3. The dot plots were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Related Product Information for anti-IL7R antibody
Interleukin 7 Receptor alpha (IL-7RA), also known as CD127, is a 75 kDa hematopoietic receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7 receptor alpha (CD127) signaling is essential for T-cell development and regulation of naive and memory T-cell homeostasis. IL-7RA is critically required for the proper development and function of lymphoid cells. Therefore, the IL-7RA is critically required for the proper development and function of lymphoid cells. Studies from both pathogenic and controlled HIV infection indicate that the containment of immune activation and preservation of CD127 expression are critical to the stability of CD4(+) T cells in infection. A better understanding of the factors regulating CD127 expression in HIV disease, particularly on T(CM) cells, might unveil new approaches exploiting the IL-7/IL-7R receptor pathway to restore T cell homeostasis and promote immune reconstitution in HIV infection. Factors relevant to HIV infection that could potentially decrease CD127 expression on human CD8(+) T cells. CD127 down-regulation may be an important contributor to HIV-associated T-cell dysfunction. In addition to IL-7, IL-7RA also associates with TSLPR to form the functional receptor for thymic stromal lymphopoietin (TSLP) which indirectly regulates T cell development by modulating dendritic cell activation. Mutations in the human IL-7RA gene cause a type of severe combined immunodeficiency in which the major deficiencies are in T cell development, whereas B and NK cells are relatively normal in number. Variation in the IL7RA gene was recently found associated with multiple sclerosis (MS). The polymorphisms in the IL7RA gene is involved in MS pathogenesis and suggest that IL7RA variation may primarily affect chronic disease courses. Soluble CD127 (sCD127) appears to play an important role in the immunopathogenesis of several chronic infections, multiple sclerosis, and various cancers.
Product Categories/Family for anti-IL7R antibody
References
Vranjkovic A, et al. (2007) IL-7 decreases IL-7 receptor alpha (CD127) expression and induces the shedding of CD127 by human CD8+ T cells. Int Immunol. 19(12): 1329-39.Kiazyk SA, et al. (2008) Loss of CD127 expression links immune activation and CD4(+) T cell loss in HIV infection. Trends Microbiol. 16(12): 567-73.Akkad DA, et al. (2009) Variation in the IL7RA and IL2RA genes in German multiple sclerosis patients. J Autoimmun. 32(2): 110-5.Crawley AM, et al. (2010) Soluble IL-7R alpha (sCD127) inhibits IL-7 activity and is increased in HIV infection. J Immunol. 184(9): 4679-87.
NCBI and Uniprot Product Information
Protein Family
Similar Products
Product Notes
The IL7R (Catalog #AAA8104456) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-IL7R alpha/CD127 Antibody (APC), Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL7R alpha/CD127 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the IL7R for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL7R alpha/CD127, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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