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Western Blot (The antibody used at 0.02-0.1ug/mL concentrations will allow visualization of 100ng/lane of recombinant human IL-8 )

Mouse IL-8 Monoclonal Antibody | anti-IL-8 antibody

mAb anti-Human IL-8

Gene Names
CXCL8; IL8
Reactivity
Human. Other species not tested
This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Protein G affinity purified
Synonyms
IL-8; Monoclonal Antibody; mAb anti-Human IL-8; Interleukin-8 (IL-8); Mouse monoclonal antibody to human interleukin 8 (IL-8); anti-IL-8 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human. Other species not tested
This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
I8-60
Specificity
This antibody recognizes both recombinant and native human Interleukin-8 (IL-8)
Purity/Purification
Protein G affinity purified
Form/Format
Lyophilized from a solution in 0.01M PBS, pH 7.0
Sequence Length
101
Applicable Applications for anti-IL-8 antibody
ELISA (EIA), NT, Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
EIA: This antibody can be used as detection antibody in sandwich ELISA applications for human IL-8 detection in combination with a monoclonal capture antibody (Cat No: MBS592159).
Neutralizing: In chemotaxis assay, the antibody inhibited 98.4% of the chemotactic activity of IL-8 on RB/293 cell using 5mg/mL of antibody and 10ng/mL of human IL-8 in assay. This antibody inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating that the antibody’s neutralization activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes.
WB: The antibody used at 0.02-0.1?g/mL concentrations will allow visualization of 100ng/lane of recombinant human IL-8.
IHC: The antibody was used to detect IL-8 expression in the stratum corneum of psoriatic skin tissue. The image below shows the result of detecting with anti-IL-8 clone 60, biotinylated 2rd antibody and avidin-HRP. The specimen was stained with DAB substrate for 5~10 min, and counter-stained with hematoxylin.
Target Protein
Human IL-8
Immunogen
Purified recombinant human IL-8
Myeloma
P3X63Ag8.653
Light Chain
Kappa
Reconstitution
Double distilled water is recommended to adjust the final concentration to 1.00mg/mL
Research Area
Cytokine, interleukin and chemotaxis
Preparation and Storage
Store at -20 degree C

Western Blot

(The antibody used at 0.02-0.1ug/mL concentrations will allow visualization of 100ng/lane of recombinant human IL-8 )

Western Blot (The antibody used at 0.02-0.1ug/mL concentrations will allow visualization of 100ng/lane of recombinant human IL-8 )

Immunohistochemistry (IHC)

(The antibody was used to detect IL-8 expression in the stratum corneum of psoriatic skin tissue. The image below shows the result of detecting with anti-IL-8 clone 60, biotinylated 2rd antibody and avidin-HRP. The specimen was stained with DAB substrate for 5~10 min, and counter-stained with hematoxylin.)

Immunohistochemistry (IHC) (The antibody was used to detect IL-8 expression in the stratum corneum of psoriatic skin tissue. The image below shows the result of detecting with anti-IL-8 clone 60, biotinylated 2rd antibody and avidin-HRP. The specimen was stained with DAB substrate for 5~10 min, and counter-stained with hematoxylin.)
Related Product Information for anti-IL-8 antibody
Interleukin 8 (IL-8), like IL-6, is secreted by macrophages and a variety of cells that express Toll-like receptors in response to the stimulation of pathogens. IL-8's primary function is to recruit neutrophils and other target cells through chemotaxis to the infected site to eliminate pathogens. IL-8 causes increased intracellular Ca2+, release of reactive oxygen species, and other physiological changes required for migration and phagocytosis. IL-8 is also known to promote angiogenesis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
interleukin-8
NCBI Official Symbol
CXCL8
NCBI Official Synonym Symbols
IL8
NCBI Protein Information
interleukin-8; C-X-C motif chemokine 8; IL-8; chemokine (C-X-C motif) ligand 8
UniProt Protein Name
Interleukin-8
UniProt Gene Name
CXCL8
UniProt Entry Name
IL8_MACMU

Uniprot Description

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus ().

Research Articles on IL-8

Similar Products

Product Notes

The IL-8 cxcl8 (Catalog #AAA592019) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The mAb anti-Human IL-8 reacts with Human. Other species not tested This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted). and may cross-react with other species as described in the data sheet. AAA Biotech's IL-8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), NT, Western Blot (WB), Immunohistochemistry (IHC). EIA: This antibody can be used as detection antibody in sandwich ELISA applications for human IL-8 detection in combination with a monoclonal capture antibody (Cat No: MBS592159). Neutralizing: In chemotaxis assay, the antibody inhibited 98.4% of the chemotactic activity of IL-8 on RB/293 cell using 5mg/mL of antibody and 10ng/mL of human IL-8 in assay. This antibody inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating that the antibody’s neutralization activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes. WB: The antibody used at 0.02-0.1?g/mL concentrations will allow visualization of 100ng/lane of recombinant human IL-8. IHC: The antibody was used to detect IL-8 expression in the stratum corneum of psoriatic skin tissue. The image below shows the result of detecting with anti-IL-8 clone 60, biotinylated 2rd antibody and avidin-HRP. The specimen was stained with DAB substrate for 5~10 min, and counter-stained with hematoxylin. Researchers should empirically determine the suitability of the IL-8 cxcl8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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