Rat anti-Human, Mouse IL-5 Monoclonal Antibody | anti-IL-5 antibody

LE/AF Purified Anti-Human/Mouse IL-5 Monoclonal Antibody

Cat. Num. AAA2559487

• Gene Names: IL5; EDF; TRF; IL-5
• Reactivity: Human, Mouse
• Applications: Neutralization Assay, Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IL-5; Monoclonal Antibody; LE/AF Purified Anti-Human/Mouse IL-5 Monoclonal Antibody; Interleukin-5; B-cell differentiation factor I; T-cell replacing factor; TRF; anti-IL-5 antibody

• Host: Rat
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: TRFK5
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: 0.2 um filtered in PBS, pH7.2. Contains no stabilizers or preservatives.
• Sequence Length: 134

• Applicable Applications for anti-IL-5 antibody: Depletion, Neutralization, Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC)
• Conjugation: None (Low endotoxin/Azide Free)
• Endotoxin: Endotoxin level is < 2 EU/mg (<0.002EU/mug) as Determined by LAL gel clotting assay.
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

Related Product Information for anti-IL-5 antibody

IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. Mouse IL-5 induces the differentiation and proliferation of pre-activated B-cells and stimulates the production and secretion of IgM and IgA by B-cells stimulated with bacterial endotoxin. The TRFK5 antibody can neutralize the bioactivity of natural or recombinant IL-5.

NCBI and Uniprot Product Information

• NCBI GI #: 4504671
• NCBI GeneID: 3567
• NCBI Accession #: NP_000870.1
• NCBI GenBank Nucleotide #: NP_000870.1
• UniProt Accession #: P05113; P04401
• NCBI Official Full Name: interleukin-5
• NCBI Official Synonym Full Names: interleukin 5
• NCBI Official Symbol: IL5
• NCBI Official Synonym Symbols: EDF; TRF; IL-5
• NCBI Protein Information: interleukin-5
• UniProt Protein Name: Interleukin-5
• UniProt Gene Name: IL5
• UniProt Synonym Gene Names: IL-5; TRF
• UniProt Entry Name: IL5_HUMAN

NCBI Description

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

Uniprot Description

IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; interleukin-5 receptor binding; growth factor activity; cytokine activity

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; positive regulation of transcription factor activity; immune response; positive regulation of immunoglobulin secretion; inflammatory response; positive regulation of eosinophil differentiation