Mouse IL-4 Monoclonal Antibody | anti-IL4 antibody

MOUSE ANTI BOVINE INTERLEUKIN-4:Biotin

Cat. Num. AAA210458

• Gene Names: IL4; IL-4; BSF-1
• Applications: ELISA, Flow Cytometry, Functional Assay
• Synonyms: IL-4; Monoclonal Antibody; MOUSE ANTI BOVINE INTERLEUKIN-4:Biotin; anti-IL4 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: CC314
• Form/Format: Biotin; Purified IgG conjugated to Biotin - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 135

• Applicable Applications for anti-IL4 antibody: ELISA (EIA), Flow cytometry (FC/FACS)*
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ; ELISA: Minimum Dilution: 1ug/ml; Maximum Dilution: 5ug/ml; Flow Cytometry: Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
• Perservative Stabilisers: 0.09% Sodium Azide; 1% Bovine Serum Albumin; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Bovine
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-IL4 antibody

Mouse anti bovine interleukin-4 antibody, clone CC314 recognizes bovine intetleukin-4 (IL-4), also known as B-cell stimulatory factor 1. IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses. Bovine interleukin-4 is a 15kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6kDa active cytokine, the apparent Mw may be altered by glycosylation (Heussler et al. 1992). IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses. Mouse anti Bovine interleukin-4, clone CC314 has been used successfully as a detection antibody in a sandwich ELISA with Clone CC313 as a coating reagent both with bovine (Stabel et al. 2011) and ovine samples (Olivier et al. 2012). Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples (Marinaro et al. 2012).

NCBI and Uniprot Product Information

• NCBI GI #: 27806871
• NCBI GeneID: 280824
• NCBI Accession #: NP_776346.1
• NCBI GenBank Nucleotide #: NM_173921.2
• UniProt Accession #: P30367; A9QWQ7
• Molecular Weight: 15,119 Da
• NCBI Official Full Name: interleukin-4
• NCBI Official Synonym Full Names: interleukin 4<
• NCBI Official Symbol: IL4
• NCBI Official Synonym Symbols: IL-4; BSF-1
• NCBI Protein Information: interleukin-4; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1
• UniProt Protein Name: Interleukin-4
• Protein Family: Interleukin
• UniProt Gene Name: IL4
• UniProt Synonym Gene Names: IL-4; BSF-1
• UniProt Entry Name: IL4_BOVIN

Uniprot Description

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages.