Mouse IL-4 Monoclonal Antibody | anti-IL4 antibody
MOUSE ANTI BOVINE INTERLEUKIN-4
Gene Names
IL4; IL-4; BSF-1
Reactivity
Goat, SheepN.B. Antibody reactivity and working conditions may vary between species.
Applications
ELISA
Synonyms
IL-4; Monoclonal Antibody; MOUSE ANTI BOVINE INTERLEUKIN-4; anti-IL4 antibody
Host
Mouse
Reactivity
Goat, Sheep
N.B. Antibody reactivity and working conditions may vary between species.
N.B. Antibody reactivity and working conditions may vary between species.
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
CC313
Specificity
IL-4
Form/Format
Purified IgG - liquid, Phosphate buffered saline
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Applicable Applications for anti-IL4 antibody
ELISA (EIA)
Application Notes
ELISA: 2ug/ml-5ug/ml
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Preservative Stabilisers
0.09% Sodium Azide
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Immunogen
Recombinant bovine IL-4
Carrier Free
Yes
Target Species
Bovine
Fusion Partners
Spleen cells form imunized BALB/c mice were fused with cells of the SP2/O myeloma cell line.
Preparation and Storage
Store at 4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Related Product Information for anti-IL4 antibody
Mouse anti Bovine interleukin-4, clone CC313 is specific for bovine interleukin 4 (IL-4), also known as B-cell stimulatory factor 1. Bovine interleukin-4 is a 15kDa, 135 amino acid peptide which is processed to a mature secreted form with the cleavage of a 24 amino acid, N-terminal signal peptide resulting in a 12.6kDa active cytokine, the apparent Mw may be altered by glycosylation (Heussler et al. 1992).
IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses.
Mouse anti Bovine interleukin-4, clone CC313 has been used successfully as a coating antibody in a sandwich ELISA with Clone CC314 as a detection reagent both with bovine (Stabel et al. 2011) and ovine samples (Olivier et al. 2012). Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples (Marinaro et al. 2012).
IL-4 is expressed by a range of cells including T and B cells, macrophages and monocytes. It plays an important role in the regulation of bovine T and B cell responses.
Mouse anti Bovine interleukin-4, clone CC313 has been used successfully as a coating antibody in a sandwich ELISA with Clone CC314 as a detection reagent both with bovine (Stabel et al. 2011) and ovine samples (Olivier et al. 2012). Clones CC313 and CC314 have been used in the reverse configuration for the measurement of interleukin-4 in caprine samples (Marinaro et al. 2012).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
interleukin-4
NCBI Official Synonym Full Names
interleukin 4
NCBI Official Symbol
IL4
NCBI Official Synonym Symbols
IL-4; BSF-1
NCBI Protein Information
interleukin-4; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1
UniProt Protein Name
Interleukin-4
Protein Family
UniProt Gene Name
IL4
UniProt Synonym Gene Names
IL-4; BSF-1
UniProt Entry Name
IL4_BOVIN
Uniprot Description
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages.
Research Articles on IL4
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Product Notes
The IL4 il4 (Catalog #AAA212744) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI BOVINE INTERLEUKIN-4 reacts with Goat, Sheep N.B. Antibody reactivity and working conditions may vary between species. and may cross-react with other species as described in the data sheet. AAA Biotech's IL-4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: 2ug/ml-5ug/ml Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Researchers should empirically determine the suitability of the IL4 il4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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