Mouse anti-Human IL-33 Monoclonal Antibody | anti-IL33 antibody
Mouse Anti-Human IL-33-UNLB
Interleukin-33 (IL-33), also known as NF-HEV, is a 30kD protein and a recently identified member of the IL-1 family. This pro-inflammatory cytokine is produced by a variety of cells, but is primarily expressed by endothelial and smooth muscle cells in the vasculature and airways2. IL-33 binds to the ST2 receptor on target cells which results in the activation of multiple signaling pathways including NF-kappaB and MAP kinases. Studies have shown that IL-33 promotes T helper type 2 (Th2) cell-mediated immune responses and upregulates production of Th2 associated cytokines including IL-4, IL-5 and IL-133,4. In vivo administration of this cytokine in mice resulted in eosinophilia and splenomegaly, as well as induced severe pathological changes in the lungs, gastrointestinal tract and other mucosal organs3,5,6
3) Schmitz et al. (2005). Immunity. 23:479-90.
4) Chackerian, A.A. et al. (2007). J. Immunol. 179:2551-5.
5) Cherry, W.B. et al. (2008). J. Aller. Clin. Immun. 121:1484-90.
6) Ho, L.H. et al. (2007). J. Leuk Biol. 82:1481-90.
NCBI and Uniprot Product Information
NCBI Description
IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM, Jan 2011]
Uniprot Description
Function: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Acts as a chemoattractant tor Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury. Ref.3 Ref.10 Ref.11 Ref.12 Ref.13 Ref.17 Ref.18In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation. Ref.3 Ref.10 Ref.11 Ref.12 Ref.13 Ref.17 Ref.18
Subunit structure: Forms a 1:1:1 heterotrimeric complex with its primary high-affinity receptor IL1RL1 and the coreceptor IL1RAP. Ref.20
Subcellular location: Nucleus. Chromosome. Cytoplasmic vesicle › secretory vesicle. Secreted. Note: Associates with heterochromatin and mitotic chromosomes. Translocation from the nucleus occurs upon biomechanical strain, depends on an intact microtubule network, and is ATP-dependent. Ref.1 Ref.3 Ref.11 Ref.12 Ref.13 Ref.17 Ref.18
Tissue specificity: Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
Domain: The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association. Ref.11
Post-translational modification: Proteolytically converted to a mature form by CASP1 in vitro and calpains in vivo. Caspase-mediated proteolysis, once thought to activate IL33, rather acts as a switch to dampen its activity. Cathepsin G and elastase can cleave IL33 and generate highly active forms in activated neutrophils (IL-33(95-270), IL-33(99-270) and IL-33(109-270)). Proteolysis is not strictly required for biological activity.
Sequence similarities: Belongs to the IL-1 family. Highly divergent.
Research Articles on IL33
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Product Notes
The IL33 il33 (Catalog #AAA670254) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human IL-33-UNLB reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-33 can be used in a range of immunoassay formats including, but not limited to, ELISA. ELISA: 1.0 - 5.0ug/mL. Researchers should empirically determine the suitability of the IL33 il33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-33, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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