Rat anti-Mouse IL-3 Monoclonal Antibody | anti-IL3 antibody

Rat Anti-Mouse IL-3-UNLB

Cat. Num. AAA670084

• Gene Names: IL3; IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF
• Reactivity: Mouse
• Applications: ELISA, Flow Cytometry, Immunofluorescence
• Synonyms: IL-3; Monoclonal Antibody; Rat Anti-Mouse IL-3-UNLB; Rat Anti-Mouse IL-3 - Purified; Unlabeled Antibody; Rat Anti-Mouse IL-3; anti-IL3 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG1 kappa
• Clone Number: MP2-8F8
• Specificity: Mouse Interleukin-3 (IL-3)1-3 (This is a neutralizing antibody.)
• Form/Format: Purified (UNLB) Antibody
• Sequence Length: 152

• Applicable Applications for anti-IL3 antibody: ELISA; Flow Cytometry; Immunofluorescence
• Application Notes: Research Application(s): ELISA Capture: MP2-8F8 monoclonal antibody is useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-3 protein levels.1-5 Purified MP2-8F8 antibody should be paired with biotinylated MP2-43D11 antibody as the detection antibody, with purified recombinant mouse IL- 3 as the standard.; Immunofluorescence/Flow Cytometry: MP2-8F8 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-3-positive cells within mixed cell populations. Recombinant mouse IL-3, unlabeled MP2-8F8, or Rat IgG1 should be used as a specificity control.; Neutralization: The LE/AF form of MP2-8F8 can be used for neutralizing mouse IL-3 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control.; Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
• Immunogen: COS-expressed recombinant mouse IL-3
• Characterization: To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.
2. Abrams, J.S., and M. Pearce. 1988. Development of rat anti-mouse interleukin 3 monoclonal antibodies which neutralize bioactivity in vitro. J. Immunol. 140:131-137.
3. Cockayne, D.A, J.S. Abrams, and A. Nienhuis. 1991. Antisense RNA inhibition of hematopoietic growth factor production. Growth Factors 5:171-181.
4. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and sera. J. Immunol. Meth. 166:201-214.
5. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP- labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.

NCBI and Uniprot Product Information

• NCBI GI #: 3002475
• NCBI GeneID: 3562
• UniProt Accession #: P08700; Q6GS87; Q6NZ78; Q6NZ79
• Molecular Weight: 17,233 Da
• NCBI Official Full Name: IL-3
• NCBI Official Synonym Full Names: interleukin 3 (colony-stimulating factor, multiple)
• NCBI Official Symbol: IL3
• NCBI Official Synonym Symbols: IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF
• NCBI Protein Information: interleukin-3; OTTHUMP00000065963; mast cell growth factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; hematopoietic growth factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor
• UniProt Protein Name: Interleukin-3
• Protein Family: Interleukin
• UniProt Gene Name: IL3
• UniProt Entry Name: IL3_HUMAN

NCBI Description

The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq]

Uniprot Description

Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Subunit structure: Monomer.

Subcellular location: Secreted.

Tissue specificity: Activated T-cells, mast cells, natural killer cells.

Sequence similarities: Belongs to the IL-3 family.