Rat anti-Mouse IL-3 Monoclonal Antibody | anti-IL3 antibody
Rat Anti-Mouse IL-3-BIOT
Gene Names
IL3; IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF
Reactivity
Mouse
Applications
ELISA, Western Blot
Synonyms
IL-3; Monoclonal Antibody; Rat Anti-Mouse IL-3-BIOT; Rat Anti-Mouse IL-3 - Biotin; Rat Anti-Mouse IL-3; anti-IL3 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a kappa
Clone Number
MP2-43D11
Specificity
Mouse Interleukin-3 (IL-3)1-4 This is a non-neutralizing antibody.
Form/Format
Biotin (BIOT) Conjugate
Sequence Length
152
Applicable Applications for anti-IL3 antibody
ELISA; Western Blot
Application Notes
Research Application(s): ELISA Detection: MP2-43D11 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying mouse IL-3 protein levels.2,3,5 Biotinylated MP2-43D11 antibody should be paired with purified MP2-8F8 antibody as the capture antibody, with purified recombinant mouse IL-3 as the standard.
Western Blotting: The purified MP2-43D11 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-3 under reducing conditions.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
Western Blotting: The purified MP2-43D11 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-3 under reducing conditions.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
Immunogen
COS-expressed recombinant mouse IL-3
Characterization
To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Reagents are stable for the period shown on the label if stored as directed.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Reagents are stable for the period shown on the label if stored as directed.
References
1. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.
2. Abrams, J.S., and M. Pearce. 1988. Development of rat anti-mouse interleukin 3 monoclonal antibodies which neutralize bioactivity in vitro. J. Immunol. 140:131-137.
3. Cockayne, D.A, J.S. Abrams, and A. Nienhuis. 1991. Antisense RNA inhibition of hematopoietic growth factor production. Growth Factors 5:171- 181.
4. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and sera. J. Immunol. Meth. 166:201-214.
5. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.
2. Abrams, J.S., and M. Pearce. 1988. Development of rat anti-mouse interleukin 3 monoclonal antibodies which neutralize bioactivity in vitro. J. Immunol. 140:131-137.
3. Cockayne, D.A, J.S. Abrams, and A. Nienhuis. 1991. Antisense RNA inhibition of hematopoietic growth factor production. Growth Factors 5:171- 181.
4. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and sera. J. Immunol. Meth. 166:201-214.
5. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
17,233 Da
NCBI Official Full Name
IL-3
NCBI Official Synonym Full Names
interleukin 3 (colony-stimulating factor, multiple)
NCBI Official Symbol
IL3
NCBI Official Synonym Symbols
IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF
NCBI Protein Information
interleukin-3; OTTHUMP00000065963; mast cell growth factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; hematopoietic growth factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor
UniProt Protein Name
Interleukin-3
Protein Family
UniProt Gene Name
IL3
UniProt Entry Name
IL3_HUMAN
NCBI Description
The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq]
Uniprot Description
Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Subunit structure: Monomer.
Subcellular location: Secreted.
Tissue specificity: Activated T-cells, mast cells, natural killer cells.
Sequence similarities: Belongs to the IL-3 family.
Research Articles on IL3
Similar Products
Product Notes
The IL3 il3 (Catalog #AAA670087) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse IL-3-BIOT reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL-3 can be used in a range of immunoassay formats including, but not limited to, ELISA; Western Blot. Research Application(s): ELISA Detection: MP2-43D11 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying mouse IL-3 protein levels.2,3,5 Biotinylated MP2-43D11 antibody should be paired with purified MP2-8F8 antibody as the capture antibody, with purified recombinant mouse IL-3 as the standard. Western Blotting: The purified MP2-43D11 antibody is also useful for Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant mouse IL-3 under reducing conditions. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications. Researchers should empirically determine the suitability of the IL3 il3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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