Rat anti-Human IL-3 Monoclonal Antibody | anti-IL3 antibody

Rat Anti-Human IL-3-UNLB

Cat. Num. AAA670033

• Gene Names: IL3; IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF
• Reactivity: Human
• Applications: ELISA, Western Blot
• Synonyms: IL-3; Monoclonal Antibody; Rat Anti-Human IL-3-UNLB; Rat Anti-Human IL-3 - Purified; Unlabeled Antibody; Rat Anti-Human IL-3; anti-IL3 antibody

• Host: Rat
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rat IgG1 kappa
• Clone Number: BVD8-3G11
• Specificity: Human Interleukin-3 (IL-3)1-4 (This is a neutralizing antibody)
• Form/Format: Purified (UNLB) Antibody
• Sequence Length: 152

• Applicable Applications for anti-IL3 antibody: ELISA; Western Blot
• Application Notes: Research Application(s): ELISA Capture: Purified BVD8-3G11 monoclonal antibody is the preferred capture antibody in a sandwich ELISA for quantifying human IL-3 protein levels.1-3 The purified BVD8-3G11 antibody should be paired with biotinylated BVD3-1F9 antibody as the detection antibody, with purified recombinant human IL-3 as the standard.; ; Western Blotting: The purified BVD8-3G11 antibody is also useful for Western blotting applications. A concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of <= 100 ng/lane of recombinant human IL-3 under reducing conditions.; ; Neutralization: The LE/AF form of BVD8-3G11 can be used for neutralizing human IL-3 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control.; ; Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
• Immunogen: Yeast-expressed recombinant human IL-3
• Characterization: To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5.
2. Abrams, J., J. Silver, R. van Dyke, and G. Gleich. 1994. Eosinophil-active cytokines in human disease: Development and use of monoclonal antibodies to IL-3-, IL-5, GM-CSF. In Eosinophils in Allergy and Inflammation (A. Kay and G. Gleich, eds). Marcel Dekker, New York, pp. 133.
3. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.
4. Kaushansky, K. 1992. J. Clin. Invest. 90:1879.

NCBI and Uniprot Product Information

• NCBI GI #: 3002475
• NCBI GeneID: 3562
• UniProt Accession #: P08700; Q6GS87; Q6NZ78; Q6NZ79
• Molecular Weight: 17,233 Da
• NCBI Official Full Name: IL-3
• NCBI Official Synonym Full Names: interleukin 3 (colony-stimulating factor, multiple)
• NCBI Official Symbol: IL3
• NCBI Official Synonym Symbols: IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF
• NCBI Protein Information: interleukin-3; OTTHUMP00000065963; mast cell growth factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; hematopoietic growth factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor
• UniProt Protein Name: Interleukin-3
• Protein Family: Interleukin
• UniProt Gene Name: IL3
• UniProt Entry Name: IL3_HUMAN

NCBI Description

The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq]

Uniprot Description

Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Subunit structure: Monomer.

Subcellular location: Secreted.

Tissue specificity: Activated T-cells, mast cells, natural killer cells.

Sequence similarities: Belongs to the IL-3 family.