Mouse anti-Human IKBKG Monoclonal Antibody | anti-IKBKG antibody

IKBKG (NF-kappa-B Essential Modulator, NEMO, NF-kappa-B Essential Modifier, Inhibitor Of Nuclear Factor kappa-B Kinase gamma Subunit, IkB Kinase gamma Subunit, I-kappa-B Kinase gamma, IKK-gamma, IKKG, IkB Kinase-associated Protein 1, IKKAP1, FIP-3, IKK ga

Cat. Num. AAA6153046

• Gene Names: IKBKG; IP; IP1; IP2; FIP3; IKKG; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; EDAID1; IKKAP1; ZC2HC9; IKK-gamma
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IKBKG; Monoclonal Antibody; IKBKG (NF-kappa-B Essential Modulator; NEMO; NF-kappa-B Essential Modifier; Inhibitor Of Nuclear Factor kappa-B Kinase gamma Subunit; IkB Kinase gamma Subunit; I-kappa-B Kinase gamma; IKK-gamma; IKKG; IkB Kinase-associated Protein 1; IKKAP1; FIP-3; IKK ga; anti-IKBKG antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4G9
• Specificity: Recognizes human IKBKG.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
• Sequence Length: 1990

• Applicable Applications for anti-IKBKG antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-420 from IKBKG (AAH50612) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MNRHLWKSQLCEMVQPSGGPAADQDVLGEESPLGKPAMLHLPSEQGAPETLQRCLEENQELRDAIRQSNQILRERCEELLHFQASQREEKEFLMCKFQEARKLVERLGLEKLDLKRQKEQALREVEHLKRCQQQMAEDKASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVVGSERK
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (72.2kD).)

Western Blot (WB)

(BKG monoclonal antibody Western Blot analysis of IKBKG expression in K-562.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to IKBKG on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to IKBKG on HeLa cell. [antibody concentration 8ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged IKBKG is ~0.1ng/ml as a capture antibody.)

Product Categories/Family for anti-IKBKG antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 34193533
• NCBI GeneID: 8517
• NCBI Official Full Name: Homo sapiens inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma, mRNA
• NCBI Official Synonym Full Names: inhibitor of nuclear factor kappa B kinase regulatory subunit gamma
• NCBI Official Symbol: IKBKG
• NCBI Official Synonym Symbols: IP; IP1; IP2; FIP3; IKKG; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; EDAID1; IKKAP1; ZC2HC9; IKK-gamma
• NCBI Protein Information: NF-kappa-B essential modulator

NCBI Description

This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016]