Mouse IkBa Monoclonal Antibody | anti-IkBa antibody

IkBa antibody (N-terminus)

Cat. Num. AAA838128

• Gene Names: NFKBIA; IKBA; MAD-3; NFKBI
• Applications: Western Blot
• Synonyms: IkBa; Monoclonal Antibody; IkBa antibody (N-terminus); Monoclonal IkBa; Anti-IkBa; I-Kappa-B; alpha; Nuclear Factor of Kappa Light polypeptide gene enhancer in B-cells inhibitor; NFKBI; Nuclear Factor of Kappa Light Chain gene enhancer in B-cells; MAD3; ikB-alpha; anti-IkBa antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: M80528
• Specificity: IkBa antibody detects endogenous levels IkBa and does not cross-react with related proteins
• Form/Format: Supplied in buffer containing 0.1M Tris-Glycine (pH 7.4, 150mM NaCl) with 0.2% sodium azide, 0.1% BSA and 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 317

• Applicable Applications for anti-IkBa antibody: Western Blot (WB)
• Biological Significance: IkBa is one member of a family of cellular proteins that function to inhibit the NF-?B transcription factor.
• Cross-Reactivity: Human
• Immunogen: IkBa antibody was raised in Rabbit using a purified recombinant human IkBaprotein fragments expressed in Ecoli as the immunogen
• Preparation and Storage: Store at 4 degree C for short term storage. For long term storage, store at -20 degree C

Western Blot (WB)

(Western blot detection of IkB-alpha(N-terminus) antibody in Jurkat&Hela lysates using IkB-alpha(N-terminus) antibody (1:1000 diluted). Predicted band size: 36KDa Observed band size: 36KDa.)

Related Product Information for anti-IkBa antibody

Mouse monoclonal IkBa antibody (N-terminus)

Product Categories/Family for anti-IkBa antibody

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 6706211
• NCBI GeneID: 4792
• UniProt Accession #: P25963; B2R8L6
• Molecular Weight: 35,609 Da
• NCBI Official Full Name: IkBa
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• NCBI Official Symbol: NFKBIA
• NCBI Official Synonym Symbols: IKBA; MAD-3; NFKBI
• NCBI Protein Information: NF-kappa-B inhibitor alpha
• UniProt Protein Name: NF-kappa-B inhibitor alpha
• UniProt Gene Name: NFKBIA
• UniProt Synonym Gene Names: IKBA; MAD3; NFKBI; IkB-alpha; IkappaBalpha
• UniProt Entry Name: IKBA_HUMAN

NCBI Description

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Uniprot Description

IkB-alpha: a regulatory protein that inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. May be involved in regulation of transcriptional responses to NF-kappa-B, including cell adhesion, immune and proinflammatory responses, apoptosis, differentiation and growth. Controlled by sequential serine-phosphorylation, ubiquitination and degradation.

Protein type: DNA-binding; Inhibitor

Chromosomal Location of Human Ortholog: 14q13

Cellular Component: cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: identical protein binding; NF-kappaB binding; protein binding; enzyme binding; ubiquitin protein ligase binding; heat shock protein binding; nuclear localization sequence binding; protein complex binding; transcription factor binding

Biological Process: nerve growth factor receptor signaling pathway; viral reproduction; apoptosis; positive regulation of cellular protein metabolic process; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; cytoplasmic sequestering of NF-kappaB; response to exogenous dsRNA; lipopolysaccharide-mediated signaling pathway; positive regulation of interferon type I production; toll-like receptor 4 signaling pathway; cytoplasmic sequestering of transcription factor; negative regulation of myeloid cell differentiation; MyD88-independent toll-like receptor signaling pathway; negative regulation of DNA binding; regulation of NF-kappaB import into nucleus; toll-like receptor 2 signaling pathway; regulation of cell proliferation; MyD88-dependent toll-like receptor signaling pathway; protein import into nucleus, translocation; inhibition of NF-kappaB transcription factor; response to muramyl dipeptide; toll-like receptor signaling pathway; innate immune response; negative regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of apoptosis

Disease: Ectodermal Dysplasia, Anhidrotic, With T-cell Immunodeficiency, Autosomal Dominant