Immunohistochemistry (IHC)
(Formalin-paraffin human tonsil stained with 172112-AF)
IgM secondary antibody
IgM (AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region, Immunoglobulin Mu Heavy Chain) (BSA & Azide Free) (MaxLight 750)
Gene Names
IGHM; MU; VH; AGM1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
IgM; Monoclonal Secondary Antibody; IgM (AGM1; IGHM; Constant Region of Heavy Chain of IgM; Ig Mu Chain C Region; Immunoglobulin Mu Heavy Chain) (BSA & Azide Free) (MaxLight 750); AGM1; Immunoglobulin Mu Heavy Chain; IgM secondary antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
SPM557
Specificity
Recognizes human IgM.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for IgM secondary antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
Application Notes
IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Recombinant heavy chain of human IgM. Cellular Localization: Cytoplasm, Cell Surface and Secreted
Conjugate
MaxLight750
Cellular Localization
Cytoplasm, Cell Surface and Secreted
Positive Control
293T, Raji or hPBL cells. Tonsil or Spleen.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunohistochemistry (IHC)
(Formalin-paraffin human tonsil stained with 172112-AF)
Immunohistochemistry (IHC)
(Formalin-paraffin human tonsil stained with 172112-AF)
Product Categories/Family for IgM secondary antibody
References
1. Maruyama S, et. al. Activation of human B cells and inhibition of their terminal differentiation by monoclonal anti-mu antibodies. Journal of Immunology 1985; 135(1):192-9. 2. Rudich SM, et. al. Human B cell activation. Evidence for diverse signals provided by various monoclonal anti-IgM antibodies. Journal of Experimental Medicine, 1985; 162(4):1236-55.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
75,000
NCBI Official Full Name
Ig mu chain C region
NCBI Official Synonym Full Names
immunoglobulin heavy constant mu
NCBI Official Symbol
IGHM
NCBI Official Synonym Symbols
MU; VH; AGM1
UniProt Protein Name
Ig mu chain C region
Protein Family
UniProt Gene Name
IGHM
UniProt Entry Name
IGHM_HUMAN
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Product Notes
The IgM ighm (Catalog #AAA6236702) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IgM (AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region, Immunoglobulin Mu Heavy Chain) (BSA & Azide Free) (MaxLight 750) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IgM can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin. IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the IgM ighm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgM, Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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