IgM secondary antibody
IgM (Immunoglobulin Mu Heavy Chain, AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region) (BSA & Azide Free) (MaxLight 650)
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
IgM; Monoclonal Secondary Antibody; IgM (Immunoglobulin Mu Heavy Chain; AGM1; IGHM; Constant Region of Heavy Chain of IgM; Ig Mu Chain C Region) (BSA & Azide Free) (MaxLight 650); Immunoglobulin Mu Heavy Chain; Ig Mu Chain C Region; IgM secondary antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
IM373
Specificity
Recognizes human IgM (Immunoglobulin Mu Heavy Chain)
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
Applicable Applications for IgM secondary antibody
FLISA, Immunohistochemistry (IHC) Frozen, Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Western Blot (WB), Immunofluorescence (IF)
Application Notes
IP: 0.5-1ug/500ug protein lysate
FC/FACS: 0.5-1ug/million cells
IHC-F: 0.5-1ug/ml for 30 minutes at RT
WB: 0.5-1ug/ml
IF: 0.5-1ug/ml
Applications are based on unconjugated antibody.
FC/FACS: 0.5-1ug/million cells
IHC-F: 0.5-1ug/ml for 30 minutes at RT
WB: 0.5-1ug/ml
IF: 0.5-1ug/ml
Applications are based on unconjugated antibody.
Immunogen
Recombinant heavy chain of human IgM. Cellular Localization: Cytoplasm, Cell Surface and Secreted
Conjugate
MaxLight650
Positive Control
293T, Raji or hPBL cells. Tonsil or Spleen.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Product Categories/Family for IgM secondary antibody
References
1. Baryshnikov AIu. Gematol Transfuziol. 1990;35(8):4-7.
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Product Notes
The IgM (Catalog #AAA6225832) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IgM (Immunoglobulin Mu Heavy Chain, AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region) (BSA & Azide Free) (MaxLight 650) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IgM can be used in a range of immunoassay formats including, but not limited to, FLISA, Immunohistochemistry (IHC) Frozen, Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Western Blot (WB), Immunofluorescence (IF). IP: 0.5-1ug/500ug protein lysate FC/FACS: 0.5-1ug/million cells IHC-F: 0.5-1ug/ml for 30 minutes at RT WB: 0.5-1ug/ml IF: 0.5-1ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the IgM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgM, Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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