IgG2b Secondary Antibody
Rat Anti-Mouse IgG2b-BIOT
Reactivity
Mouse
Applications
Flow Cytometry, ELISA
Synonyms
IgG2b; Monoclonal Secondary Antibody; Rat Anti-Mouse IgG2b-BIOT; Rat Anti-Mouse IgG2b (gamma2b chain specific); IgG2b secondary antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
SB74g
Specificity
Reacts with the gamma2b heavy chain of mouse IgG2b. Minimal reaction with other mouse immunoglobulins as determined by ELISA. May react with immunoglobulins from other species.
Form/Format
Biotin (BIOT) Conjugate
Applicable Applications for IgG2b secondary antibody
Identification and enumeration of IgG2b+ cells by Flow Cytometry, Second step reagent for mouse IgG2b monoclonal antibodies, ELISA
Application Notes
Flow Cytometry: <=1 ug/10^6 cells
ELISA: 1:5,000-1:20,000
ELISA: 1:5,000-1:20,000
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by ELISA and/or flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Preparation and Storage
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C
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Product Notes
The IgG2b (Catalog #AAA675069) is a Secondary Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse IgG2b-BIOT reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IgG2b can be used in a range of immunoassay formats including, but not limited to, Identification and enumeration of IgG2b+ cells by Flow Cytometry, Second step reagent for mouse IgG2b monoclonal antibodies, ELISA. Flow Cytometry: <=1 ug/10^6 cells ELISA: 1:5,000-1:20,000. Researchers should empirically determine the suitability of the IgG2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG2b, Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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