Western Blot (WB)
(Fig. Western blot analysis ( lane 1: 1:10000, lane 2: 1:20000, lane 3: 1:40000, lane 4: 1:80000 ) using IPKine HRP, Mouse Anti-Rabbit IgG HCS.)
IgG secondary antibody
IPKine HRP, Mouse Anti-Rabbit IgG HCS
Reactivity
Rabbit
Applications
Western Blot, Immunoprecipitation
Purity
Affinity purified using solid phase Rabbit IgG(H+L) light chain with finally >95% purity based on SDS-PAGE
Synonyms
IgG; Monoclonal Secondary Antibody; IPKine HRP; Mouse Anti-Rabbit IgG HCS; Mouse Anti-Rabbit IgG; IgG secondary antibody
Host
Mouse
Reactivity
Rabbit
Clonality
Monoclonal
Isotype
Mouse IgG
Purity/Purification
Affinity purified using solid phase Rabbit IgG(H+L) light chain with finally >95% purity based on SDS-PAGE
Form/Format
Liquid in PBS, pH7.4, containing 1% BSA as stablizer and 50% Glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for IgG secondary antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
The suggested dilution ratio is :
WB: 1:1000-1:10,000 (1:2000 optimal)
IP: 1:1000-1:10,000 (1:2000 optimal)
Note: Contain sodium azide.
WB: 1:1000-1:10,000 (1:2000 optimal)
IP: 1:1000-1:10,000 (1:2000 optimal)
Note: Contain sodium azide.
Immunogen
Rabbit IgG heavy chain
Conjugation
HRP
Features & Benefits
50kD or 25kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination). IPKine products solve these problems through less background noise, and enhanced accuracy). Their light chain/Fc fragment specific optimization will eliminate heavy/light chain interference, while serum proteins absorbed minimize cross-reactivity with immunoglobulins from other species which may be present on blots).
Preparation and Storage
Stable for one year at -20 degree C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Western Blot (WB)
(Fig. Western blot analysis ( lane 1: 1:10000, lane 2: 1:20000, lane 3: 1:40000, lane 4: 1:80000 ) using IPKine HRP, Mouse Anti-Rabbit IgG HCS.)
Western Blot (WB)
(Fig. Western blot analysis ( lane 1: 1:10000, lane 2: 1:20000, lane 3: 1:40000, lane 4: 1:80000 ) using IPKine HRP, Mouse Anti-Rabbit IgG HCS.)
Related Product Information for IgG secondary antibody
Background: Secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application.
Product Categories/Family for IgG secondary antibody
Similar Products
Product Notes
The IgG (Catalog #AAA9718989) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IPKine HRP, Mouse Anti-Rabbit IgG HCS reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's IgG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). The suggested dilution ratio is : WB: 1:1000-1:10,000 (1:2000 optimal) IP: 1:1000-1:10,000 (1:2000 optimal) Note: Contain sodium azide. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG, Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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