IGF-1 Clone 1F-3H10 Monoclonal Antibody
Anti-human Insulin-like Growth Factor 1 Monoclonal Antibody
Reactivity
HumanCrossreactivity Note: No cross reaction with insulin or IGF-2
Applications
ELISA, Immunoblot, Western Blot, Western Blot
Synonyms
IGF-1 Clone 1F-3H10; Monoclonal Antibody; Anti-human Insulin-like Growth Factor 1 Monoclonal Antibody; anti-IGF-1 Clone 1F-3H10 antibody
Reactivity
Human
Crossreactivity Note: No cross reaction with insulin or IGF-2
Crossreactivity Note: No cross reaction with insulin or IGF-2
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
1F-3H10
Specificity
Polypeptide ::: % Cross Reactivity
hIGF-1 ::: 100
hIGF-1 ::: 100
Form/Format
Supplied as Ascites Fluid (sterile filtered)
Sequence Length
71
Applicable Applications for anti-IGF-1 Clone 1F-3H10 antibody
Indirect ELISA, Western Immunoblot, Western Blot, Immunofluorescent Staining
Application Notes
ELISA: This monoclonal antibody containing ascites fluid has been found by indirect ELISA to bind to hIGF-1. and titration experiments show the titer to be greater than 1:5000.
Western Blot: Western immunoblots resulted in a single band being detected at ~ 7.5 kDa at a dilution of 1:5000
Immunofluorescent: This monoclonal antibody has been found to stain cells which produce IGF-1 at a 1:2000 dilution. The cultured cells were washed x 4 and fixed with neutral buffered formalin
Immunofluorescent: This monoclonal antibody has been found to stain cells which produce IGF-1 at a 1:2000 dilution. The cultured cells were washed x 4 and fixed with neutral buffered formalin
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the mouse ascites after dilution to at least 1:5000 (Note: higher dilutions may be needed). Use 1% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the antibody bind for 1 - 2 hours.
4. Wash x 4 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-mouse IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 1% normal goat serum in TBS/Tween-20. Incubate 1 - 2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 6 for at least 5 min per wash cycle in TBS/Tween-20.
7. Develop color using the enhanced DAB reaction
Western Blot: Western immunoblots resulted in a single band being detected at ~ 7.5 kDa at a dilution of 1:5000
Immunofluorescent: This monoclonal antibody has been found to stain cells which produce IGF-1 at a 1:2000 dilution. The cultured cells were washed x 4 and fixed with neutral buffered formalin
Immunofluorescent: This monoclonal antibody has been found to stain cells which produce IGF-1 at a 1:2000 dilution. The cultured cells were washed x 4 and fixed with neutral buffered formalin
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the mouse ascites after dilution to at least 1:5000 (Note: higher dilutions may be needed). Use 1% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the antibody bind for 1 - 2 hours.
4. Wash x 4 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-mouse IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 1% normal goat serum in TBS/Tween-20. Incubate 1 - 2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 6 for at least 5 min per wash cycle in TBS/Tween-20.
7. Develop color using the enhanced DAB reaction
Related Product Information for anti-IGF-1 Clone 1F-3H10 antibody
This ascites fluid contains mouse monoclonal antibody 1F6-3H10 raised against recombinant hIGF-1. This monoclonal has been found to bind specifically to hIGF-1 by indirect ELISA. western immunoblots. and immunocytochemistry. This monoclonal antibody was tested for recognition of insulin. IGF-2. and other growth factors by ELISA techniques. It has been found to be a mouse IgG1 Kappa by isotyping
Product Categories/Family for anti-IGF-1 Clone 1F-3H10 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
IGF-1
Similar Products
Product Notes
The IGF-1 Clone 1F-3H10 (Catalog #AAA350013) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-human Insulin-like Growth Factor 1 Monoclonal Antibody reacts with Human Crossreactivity Note: No cross reaction with insulin or IGF-2 and may cross-react with other species as described in the data sheet. AAA Biotech's IGF-1 Clone 1F-3H10 can be used in a range of immunoassay formats including, but not limited to, Indirect ELISA, Western Immunoblot, Western Blot, Immunofluorescent Staining. ELISA: This monoclonal antibody containing ascites fluid has been found by indirect ELISA to bind to hIGF-1. and titration experiments show the titer to be greater than 1:5000. Western Blot: Western immunoblots resulted in a single band being detected at ~ 7.5 kDa at a dilution of 1:5000 Immunofluorescent: This monoclonal antibody has been found to stain cells which produce IGF-1 at a 1:2000 dilution. The cultured cells were washed x 4 and fixed with neutral buffered formalin Immunofluorescent: This monoclonal antibody has been found to stain cells which produce IGF-1 at a 1:2000 dilution. The cultured cells were washed x 4 and fixed with neutral buffered formalin Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer. 2. Wash x 2 with TBS/Tween-20. 3. Apply the mouse ascites after dilution to at least 1:5000 (Note: higher dilutions may be needed). Use 1% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the antibody bind for 1 - 2 hours. 4. Wash x 4 with TBS/Tween-20. 5. Apply affinity purified HRP-goat anti-mouse IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 1% normal goat serum in TBS/Tween-20. Incubate 1 - 2 hours. Note: greater sensitivity may be achieved using ABC techniques. 6. Wash x 6 for at least 5 min per wash cycle in TBS/Tween-20. 7. Develop color using the enhanced DAB reaction. Researchers should empirically determine the suitability of the IGF-1 Clone 1F-3H10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IGF-1 Clone 1F-3H10, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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