Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Lymph Node stained with IgD Mouse Monoclonal Antibody (IGD26).)
Mouse anti-Human IgD (Immunoglobulin Delta Heavy Chain) Monoclonal Antibody | anti-IGHD antibody
IgD (Immunoglobulin Delta Heavy Chain) (B-Cell Marker)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
IgD (Immunoglobulin Delta Heavy Chain); Monoclonal Antibody; IgD (Immunoglobulin Delta Heavy Chain) (B-Cell Marker); Ig delta chain C region; IGHD; Immunoglobulin heavy constant delta; anti-IGHD antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
IgD26
Specificity
Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through interchain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin D (IgD) exists as a monomer with light chains. It plays a biological role as a transmembrane receptor molecule, co-expressed with IgM on the surface of mature/naive B cells. In particular, it is found on spleen B cell surfaces. Compared to IgM, IgD exists in much lower numbers and is not expressed on immature B cells. IgD surface expression on B cells is regulated in part by IL-27. In mice, the inhibition of this immunoglobulin isotype does not cause a significant change to the immune system.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
384
Applicable Applications for anti-IGHD antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
Application Notes
FC/FACS: 0.5-1ug/million cells
IF: 0.5-1ug/ml
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)
IF: 0.5-1ug/ml
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)
Positive Control
Human tonsil lysate; NAMALWA cell lysate; Human tonsil and lymphoid tissues.
Immunogen
Full-length human IGHD protein
Cellular Localization
Cytoplasmic
Hu-chromosome Location
14q13
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Lymph Node stained with IgD Mouse Monoclonal Antibody (IGD26).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Lymph Node stained with IgD Mouse Monoclonal Antibody (IGD26).)
SDS-Page
(SDS-PAGE Analysis Purified IgD Mouse Monoclonal Antibody (IGD26). Confirmation of Purity and Integrity of Antibody.)
SDS-Page
(SDS-PAGE Analysis Purified IgD Mouse Monoclonal Antibody (IGD26). Confirmation of Purity and Integrity of Antibody.)
References
Takayasu, T., et al. 1980. Biochem. Biophys. Res. Commun. 97: 635-641.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
44-80kDa (heavy chain); 21-25kDa (kappa light chain)
NCBI Official Full Name
Ig delta chain C region
UniProt Protein Name
Ig delta chain C region
Protein Family
UniProt Gene Name
IGHD
UniProt Entry Name
IGHD_HUMAN
Uniprot Description
IGHD: IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. Soluble IgD (sIgD) concentration in serum below those of IgG, IgA, and IgM but much higher than that of IgE. IgM and IgD molecules present on B cells have identical V regions and antigen-binding sites. After the antigen binds to the B-cell receptor, the secreted form sIgD is shut off. IgD is a potent inducer of TNF, IL1B, and IL1RN. IgD also induces release of IL6, IL10, and LIF from peripheral blood mononuclear cells. Monocytes seem to be the main producers of cytokines in vitro in the presence of IgD. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 14q32.33
Cellular Component: plasma membrane; integral to membrane
Molecular Function: antigen binding
Biological Process: immune response
Similar Products
Product Notes
The IGHD ighd (Catalog #AAA4380996) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IgD (Immunoglobulin Delta Heavy Chain) (B-Cell Marker) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IgD (Immunoglobulin Delta Heavy Chain) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin. FC/FACS: 0.5-1ug/million cells IF: 0.5-1ug/ml Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the IGHD ighd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgD (Immunoglobulin Delta Heavy Chain), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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