Mouse ID3 Monoclonal Antibody | anti-ID3 antibody

Mouse monoclonal ID3 antibody

Cat. Num. AAA5306427

• Gene Names: ID3; HEIR-1; bHLHb25
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: ID3; Monoclonal Antibody; Mouse monoclonal ID3 antibody; ID3 antibody; anti-ID3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 15F4
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 119

• Applicable Applications for anti-ID3 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC: 1:100; IF: 1:100; IHC: 1:50; WB: 1:2000
• Immunogen: ID3 antibody was raised in mouse using a full length recombinant protein of human ID3 (NP_002158) produced in HEK293T cells, as the immunogen.
• Cross-Reactivity: Human
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Hela cells using ID3 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Product Categories/Family for anti-ID3 antibody

DNA & RNA

NCBI and Uniprot Product Information

• NCBI GI #: 313213
• NCBI GeneID: 3399
• UniProt Accession #: Q02535; O75641; A8K1T8
• Molecular Weight: 12,999 Da
• NCBI Official Full Name: Id3
• NCBI Official Synonym Full Names: inhibitor of DNA binding 3, HLH protein
• NCBI Official Symbol: ID3
• NCBI Official Synonym Symbols: HEIR-1; bHLHb25
• NCBI Protein Information: DNA-binding protein inhibitor ID-3
• UniProt Protein Name: DNA-binding protein inhibitor ID-3
• Protein Family: DNA-binding protein inhibitor
• UniProt Gene Name: ID3
• UniProt Synonym Gene Names: 1R21; BHLHB25; HEIR1; bHLHb25

NCBI Description

The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with other HLH proteins. However, the encoded protein lacks a basic DNA-binding domain and therefore inhibits the DNA binding of any HLH protein with which it interacts. [provided by RefSeq, Aug 2011]

Uniprot Description

ID3: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors. Induced by phorbol 12-myristate 13-acetate (PMA). Interacts with COPS5 and COPS7A. Homodimer, and heterodimer with other HLH proteins.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: microtubule cytoskeleton; nucleoplasm; nucleus

Molecular Function: protein binding; transcription corepressor activity

Biological Process: multicellular organismal development; negative regulation of transcription factor activity; negative regulation of transcription, DNA-dependent