Rabbit anti-Human ICAD Monoclonal Antibody | anti-ICAD antibody

ICAD Rabbit mAb

Cat. Num. AAA4753308

• Gene Names: DFFA; DFF1; ICAD; DFF-45
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: ICAD; Monoclonal Antibody; ICAD Rabbit mAb; DFF1; DFF-45; anti-ICAD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 50nM Tris-Glycine (pH7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA

• Applicable Applications for anti-ICAD antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:2000-1:10,000; IP: 1:20
• Immunogen: A synthetic peptide of human ICAD
• Conjugation: Unconjugated
• Modification: Unmodification
• Preparation and Storage: Store at 4 degree C short term. Store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of ICAD in A549,HL-60,U2OS cell lysates using ICAD Rabbit mAb(1:1000 diluted). Predicted band size:37kDa. Observed band size:45kDa.)

Related Product Information for anti-ICAD antibody

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

NCBI and Uniprot Product Information

• NCBI GI #: 4758148
• NCBI GeneID: 1676
• NCBI Accession #: NP_004392.1
• NCBI GenBank Nucleotide #: NM_004401.2
• UniProt Accession #: O00273; Q5T6G5; Q5T6G6; Q96I97; Q9Y6C6
• Molecular Weight: 36,522 Da
• NCBI Official Full Name: DNA fragmentation factor subunit alpha isoform 1
• NCBI Official Synonym Full Names: DNA fragmentation factor, 45kDa, alpha polypeptide
• NCBI Official Symbol: DFFA
• NCBI Official Synonym Symbols: DFF1; ICAD; DFF-45
• NCBI Protein Information: DNA fragmentation factor subunit alpha; DFF45; inhibitor of CAD; DNA fragmentation factor 45 kDa subunit
• UniProt Protein Name: DNA fragmentation factor subunit alpha
• Protein Family: Poly-beta-1,6-N-acetyl-D-glucosamine synthesis protein
• UniProt Gene Name: DFFA
• UniProt Synonym Gene Names: DFF1; DFF45; DFF-45; ICAD
• UniProt Entry Name: DFFA_HUMAN

NCBI Description

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DFFA: Inhibitor of the caspase-activated DNase (DFF40). Heterodimer of DFFA and DFFB. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase

Chromosomal Location of Human Ortholog: 1p36.3-p36.2

Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; lipid particle; nucleus; cytosol

Molecular Function: protein binding

Biological Process: positive regulation of apoptosis; apoptosis; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis