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Mouse Human Procollagen Type I C-Peptide (PIP) Monoclonal Antibody

Human Procollagen Type I C-Peptide (PIP) Monoclonal Antibody

Applications
ELISA, Western Blot, Immunohistochemistry
Synonyms
Human Procollagen Type I C-Peptide (PIP); Monoclonal Antibody; Human Procollagen Type I C-Peptide (PIP) Monoclonal Antibody; Procollagen Type I C-Peptide (PIP); anti-Human Procollagen Type I C-Peptide (PIP) antibody
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For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
These antibodies recognize non-denatured human, bovine, canine, and equine Procollagen Type I C-peptide. They do not react with rat or rabbit Procollagen Type 1 C-peptide.
Form/Format
100 ug IgG in 10mM PBS, pH 7.4, 1% BSA, lyophilized.
Applicable Applications for anti-Human Procollagen Type I C-Peptide (PIP) antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
ELISA: 0.2ug/ml with solid phase antigen. For sandwich ELISA, use MBS190258 on the solid phase and labeled MBS190258 for detection of binding.

Western Blot: 10ug/ml under non-reducing and non-heating conditions.

Immunohistochemistry: 10ug/ml on cultured cells, frozen and paraffin-embedded tissue sections (Antigen retrieval: Proteinase K treatment)

Dissolve lyophilized antibody in 50 uL of distilled water (final concentration 2.0 mg/mL).
Antigen
Human Procollagen Type I C-Peptide.
Apperance
White Powder
Preparation and Storage
This product does not contain preservative. The stock solution (2.0mg/ml) can be stored in aliquots at -20°C for 1 year or can be stored at 4°C for 6 months after adding 0.1% sodium azide. Dilutions of stock solution should not be stored. Avoid repeated freeze-thaw cycles.
Related Product Information for anti-Human Procollagen Type I C-Peptide (PIP) antibody
Collagens (types I, II, III, IV, and V) are synthesized as precursor molecules called procollagens. These contain additional peptide sequences, the “propeptides”, at the amino- and carboxy- terminal ends. Propeptides facilitate the winding of procollagen molecules into a triple-helix conformation within the endoplasmic reticulum. These propeptides are cleaved from the collagen triple helix during its secretion after which the collagens polymerize into extracellular collagen fibrils. Therefore, the amount of free propeptides correlates directly with collagen molecule synthesis. Procollagen Type I C-peptide (PIP) has been extensively referenced in studies that correlated collagen levels and disorders such as bone disease, alcoholic liver disease, liver cirrhosis, and scirrhous (Borrmann type IV) adenocarcinoma of the stomach.
Product Categories/Family for anti-Human Procollagen Type I C-Peptide (PIP) antibody

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Product Notes

The Human Procollagen Type I C-Peptide (PIP) (Catalog #AAA190258) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Human Procollagen Type I C-Peptide (PIP) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. ELISA: 0.2ug/ml with solid phase antigen. For sandwich ELISA, use MBS190258 on the solid phase and labeled MBS190258 for detection of binding. Western Blot: 10ug/ml under non-reducing and non-heating conditions. Immunohistochemistry: 10ug/ml on cultured cells, frozen and paraffin-embedded tissue sections (Antigen retrieval: Proteinase K treatment) Dissolve lyophilized antibody in 50 uL of distilled water (final concentration 2.0 mg/mL). Researchers should empirically determine the suitability of the Human Procollagen Type I C-Peptide (PIP) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Human Procollagen Type I C-Peptide (PIP), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.
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