Western Blot (WB)
(Western Blot detection against Immunogen (44.2kD).)
Mouse anti-Human HSPB6 Monoclonal Antibody | anti-HSPB6 antibody
HSPB6 (Heat Shock Protein beta-6, Heat Shock 20kD-like Protein p20, FLJ32389, Hsp20) APC
Gene Names
HSPB6; HEL55; Hsp20; PPP1R91
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
HSPB6; Monoclonal Antibody; HSPB6 (Heat Shock Protein beta-6; Heat Shock 20kD-like Protein p20; FLJ32389; Hsp20) APC; anti-HSPB6 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, lambda
Clone Number
6A4
Specificity
Recognizes human HSPB6.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Sequence Length
1463
Applicable Applications for anti-HSPB6 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant protein corresponding to aa1-160 from human HSPB6 (AAH68046) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MEIPVPVQPSWLRRASAPLLGLSAPGRLFDQRFGEGLLEAELAALCPTTLAPYYLRAPSVALPVAQVPTDPGHFSVLLDVKHFSPEEIAVKVVGEHVEVHARHEERPDEHGFVAREFHRRYRLPPGVDPAAVTSALSPEGVLSIQAAPASAQAPPPAAAK
Conjugate
APC
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (44.2kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (44.2kD).)
Western Blot (WB)
(Western Blot analysis of HSPB6 expression in transfected 293T cell line by HSPB6 monoclonal antibody. Lane 1: HSPB6 transfected lysate (17.2kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of HSPB6 expression in transfected 293T cell line by HSPB6 monoclonal antibody. Lane 1: HSPB6 transfected lysate (17.2kD). Lane 2: Non-transfected lysate.)
Testing Data
(Detection limit for recombinant GST tagged HSPB6 is 0.3ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged HSPB6 is 0.3ng/ml as a capture antibody.)
Related Product Information for anti-HSPB6 antibody
HSPB6 is associated with actin (see MIM 102540) and modulates smooth muscle relaxation (Tessier et al., 2003 [PubMed 12842460]).
Product Categories/Family for anti-HSPB6 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Homo sapiens heat shock protein, alpha-crystallin-related, B6, mRNA
NCBI Official Synonym Full Names
heat shock protein family B (small) member 6
NCBI Official Symbol
HSPB6
NCBI Official Synonym Symbols
HEL55; Hsp20; PPP1R91
NCBI Protein Information
heat shock protein beta-6
Protein Family
NCBI Description
This locus encodes a heat shock protein. The encoded protein likely plays a role in smooth muscle relaxation. [provided by RefSeq, Jan 2012]
Research Articles on HSPB6
Similar Products
Product Notes
The HSPB6 (Catalog #AAA6137073) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HSPB6 (Heat Shock Protein beta-6, Heat Shock 20kD-like Protein p20, FLJ32389, Hsp20) APC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSPB6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HSPB6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPB6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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