Western Blot (WB)
(Western Blot analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody at 1:1000.)
Mouse Hsp70 Monoclonal Antibody | anti-HSPA1A antibody
GIT1 Antibody: FITC
Gene Names
HSPA1A; HSPA1; HSP70-1
Reactivity
Human, Bovine, Mouse, Rat, Porcine
Applications
Western Blot, ELISA
Synonyms
Hsp70; Monoclonal Antibody; GIT1 Antibody: FITC; Hsp70.1; Hsp70.2; Hsp72; HSPA1; HSPA1A; HSPA1B; anti-HSPA1A antibody
Host
Mouse
Reactivity
Human, Bovine, Mouse, Rat, Porcine
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1.86
Form/Format
Protein G Affinity Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
641
Applicable Applications for anti-HSPA1A antibody
Western Blot (WB), ELISA (EIA)
Application Notes
1ug/ml WB
Conjugate
FITC
Storage Buffer
PBS pH7.2, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Western Blot (WB)
(Western Blot analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody at 1:1000.)
Western Blot (WB)
(Western Blot analysis of Rat brain membrane lysate showing detection of GIT1 protein using Mouse Anti-GIT1 Monoclonal Antibody, Clone S39B-8. Primary Antibody: Mouse Anti-GIT1 Monoclonal Antibody at 1:1000.)
Related Product Information for anti-HSPA1A antibody
Background Info: Detects a 70kDa protein corresponding to the molecular mass of inducible hsp70 on SDS Page immunoblots. Does not cross-react with Hsc70.
Scientific Background: G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin (reviewed in 1). GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration (2). GIT-1 has also been implicated in neuronal functions including synapse formation (3) and the pathology of Huntington disease (4). Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains (5). Htt interacts directly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology (4).
Scientific Background: G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin (reviewed in 1). GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration (2). GIT-1 has also been implicated in neuronal functions including synapse formation (3) and the pathology of Huntington disease (4). Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains (5). Htt interacts directly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology (4).
Product Categories/Family for anti-HSPA1A antibody
References
1. Hoefen R.J. and Berk B.C. (2006) J. Cell Sci. 119: 146 1475. 2. 2. Manabe R., et al. (2002) J. Cell Sci. 115: 1497-1510. 3. 3. Zhang H., et al. (2003) J. Cell Biol. 161: 131-142. 4. 4. Goehler H., et al. (2004) Mol. Cell 15: 853-865. 5. 5. Mende-Mueller L.M., et al. (2001) J. Neurosci. 21: 1830-1837.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70,259 Da
NCBI Official Full Name
heat shock 70 kDa protein 1A
NCBI Official Symbol
HSPA1A
NCBI Official Synonym Symbols
HSPA1; HSP70-1
NCBI Protein Information
heat shock 70 kDa protein 1A; HSP70.1; heat shock 70 kD protein 1; heat-shock 70-kilodalton protein 1A
UniProt Protein Name
Heat shock 70 kDa protein 1A
Protein Family
UniProt Gene Name
HSPA1A
UniProt Synonym Gene Names
HSP70-1; HSP70.1
UniProt Entry Name
HS71A_BOVIN
Uniprot Description
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation.
Research Articles on HSPA1A
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Product Notes
The HSPA1A hspa1a (Catalog #AAA804918) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GIT1 Antibody: FITC reacts with Human, Bovine, Mouse, Rat, Porcine and may cross-react with other species as described in the data sheet. AAA Biotech's Hsp70 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). 1ug/ml WB. Researchers should empirically determine the suitability of the HSPA1A hspa1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp70, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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