Mouse anti-Human HSP60 Monoclonal Antibody | anti-HSP60 antibody

HSP60 (Heat Shock Protein 60, 60kD chaperonin, 60kD heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Short heat shock protein 60 Hsp60s1, Spastic para

Cat. Num. AAA6193799

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot, Immunofluorescence, Immunoprecipitation
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: HSP60; Monoclonal Antibody; HSP60 (Heat Shock Protein 60; 60kD chaperonin; 60kD heat shock protein mitochondrial; Chaperonin; 60-KD (CPN60); GROEL; HLD4; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; Short heat shock protein 60 Hsp60s1; Spastic para; Heat Shock Protein 60; 60kDa chaperonin; 60kDa heat shock protein mitochondrial; Spastic paraplegia 13 (SPG13); anti-HSP60 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: LK1
• Specificity: Recognizes human HSP60. Species Crossreactivity: mouse, rat, hamster, sheep, rabbit, bovine, canine, porcine, monkey, chicken, xenopus, and drosophila. Does not react with bacteria, helminths, or spinach.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-HSP60 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC) Frozen/Paraffin, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: FC/FACS: 0.5-1ug/million cells; IHC-F/P: 0.5-1ug/ml for 30 minutes at RT; IP: 0.5-1ug/500ug protein lysate; IF: 0.5-1ug/ml; WB: 0.5-1ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Recombinant human HSP60 protein. Cellular Localization: Mitochondria in cytoplasm
• Conjugate: MaxLight405
• Positive Control: HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Product Categories/Family for anti-HSP60 antibody

Antibodies; Heat Shock Proteins

References

1. Boog CJ; de Graeff-Meeder ER; Lucassen MA; van der Zee R; Voorhorst-Ogink MM; van Kooten PJ; Geuze HJ; van Eden W. Two monoclonal antibodies generated against human hsp60 show reactivity with synovial membranes of patients with juvenile chronic arthritis. Journal of Experimental Medicine, 1992, 175(6):1805-10.