Mouse Hsp60 Monoclonal Antibody
Anti-Hsp60 Monoclonal Antibody
Applications
Flow Cytometry, Immunoblot, Western Blot, Immunohistochemistry
Synonyms
Hsp60; Monoclonal Antibody; Anti-Hsp60 Monoclonal Antibody; Anti-Hsp60; anti-Hsp60 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
LK-2
Specificity
Anti Hsp60 [Mouse monoclonal antibody to human Hsp60]
This antibody recognizes human, mouse, rat, rabbit, bovine, canine, porcine, guinea pig, hamster, chicken, monkey, white fly, yeast and bacterial* Hsp60 (60 kDa). The epitope recognized is within aa 383-419 of human Hsp60.
Borellia, E. coli, Helicobacter pylori, M. bovis, Salmonella typhimurium, Streptococcus pyogenes, Treponema hyodysenteriae, Treponema innocense, Trichinella spiralis, Yersinia enterocolitica.
This antibody recognizes human, mouse, rat, rabbit, bovine, canine, porcine, guinea pig, hamster, chicken, monkey, white fly, yeast and bacterial* Hsp60 (60 kDa). The epitope recognized is within aa 383-419 of human Hsp60.
Borellia, E. coli, Helicobacter pylori, M. bovis, Salmonella typhimurium, Streptococcus pyogenes, Treponema hyodysenteriae, Treponema innocense, Trichinella spiralis, Yersinia enterocolitica.
Form/Format
100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
Applicable Applications for anti-Hsp60 antibody
Flow Cytometry, Immunoblot, Immunohistochemistry
Application Notes
Immunoblotting: use at 0.25-1ug/ml. A band of ~60 kDa is detected.
Immunohistochemistry: use at 5ug/ml
Flow cytometry: use at 10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Heat-shocked HeLa cell lysate.
Immunohistochemistry: use at 5ug/ml
Flow cytometry: use at 10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Heat-shocked HeLa cell lysate.
Antigen
Recombinant human Hsp60 expressed in E. coli.
Preservatives
0.09% sodium azide
Other Additives
50% glycerol
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.
Related Product Information for anti-Hsp60 antibody
Hsp60 is an abundant protein synthesized constitutively in various cell types that is induced to higher concentrations after cell shock. It is present in mitochondria of many mammalian species and has highly similar counterparts in bacteria and plants (where is localized to chloroplasts). In general, Hsp60 proteins are present in high concentrations, are induced in response to environmental stresses (such as heat shock) are homo-oligomeric structures of 7 or 14 subunits that dissociate reversibly in the presence of Mg2+ and ATP, have ATPase activity, and play a role in folding and assembly of oligomeric protein structures. Hsp60 has been linked to Alzheimer's disease, coronary artery disease, multiple sclerosis, diabetes, and other autoimmune diseases.
Product Categories/Family for anti-Hsp60 antibody
NCBI and Uniprot Product Information
Uniprot Description
Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.
Similar Products
Product Notes
The Hsp60 (Catalog #AAA190474) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Hsp60 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry, Immunoblot, Immunohistochemistry. Immunoblotting: use at 0.25-1ug/ml. A band of ~60 kDa is detected. Immunohistochemistry: use at 5ug/ml Flow cytometry: use at 10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Heat-shocked HeLa cell lysate. Researchers should empirically determine the suitability of the Hsp60 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp60, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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