Rabbit anti-Human HSP27  Monoclonal Antibody | anti-HSP27  antibody

Rabbit anti Human HSP27  Monoclonal Antibody

Cat. Num. AAA2543950

• Gene Names: HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Synonyms: HSP27 ; Monoclonal Antibody; Rabbit anti Human HSP27 Monoclonal Antibody; anti-HSP27 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 205

• Applicable Applications for anti-HSP27 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 0.5-2mug/mL; ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human HSP27. The detection limit for Human HSP27 is approximately 0.00245 ng/well.; IP: 1-4 muL/mg of lysate
• Immunogen: Recombinant Human HSP27 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 11036357
• NCBI GeneID: 3315
• Molecular Weight: 22,783 Da
• NCBI Official Full Name: HSP27
• NCBI Official Synonym Full Names: heat shock protein family B (small) member 1
• NCBI Official Symbol: HSPB1
• NCBI Official Synonym Symbols: CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
• NCBI Protein Information: heat shock protein beta-1
• UniProt Protein Name: Heat shock protein beta-1
• UniProt Gene Name: HSPB1
• UniProt Synonym Gene Names: HSP27; HSP28; HspB1; HSP 27; SRP27
• UniProt Entry Name: HSPB1_HUMAN

NCBI Description

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]

Uniprot Description

HSP27: a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.

Protein type: Chaperone; Heat shock protein; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: cytoplasm; cytoskeleton; cytosol; extracellular matrix; extracellular space; focal adhesion; nucleus; proteasome complex

Molecular Function: identical protein binding; protein binding; protein kinase binding; protein kinase C binding; protein kinase C inhibitor activity; ubiquitin binding

Biological Process: cell motility; negative regulation of apoptosis; negative regulation of protein kinase activity; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of interleukin-1 beta production; positive regulation of tumor necrosis factor biosynthetic process; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of mRNA stability; regulation of protein amino acid phosphorylation; regulation of translational initiation; response to virus; retinal homeostasis; vascular endothelial growth factor receptor signaling pathway

Disease: Charcot-marie-tooth Disease, Axonal, Type 2f; Neuronopathy, Distal Hereditary Motor, Type Iib